Recombinant Mouse Anti-BRAF Antibody (clone 2A10D7) (CAT#: VS3-FY165)
This product is a recombinant mouse antibody that recognizes BRAF protein. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg
(Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg
Figure 5 Colon
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- BRAF
- Clone
- 2A10D7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95%
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- B-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma Viral Oncogene Homolog B1; V-Raf Murine Sarcoma Viral Oncogene Homolog B; Proto-Oncogene B-Raf; BRAF1; RAFB1; B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94); Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1; Serine/Threonine-Protein Kinase B-Raf
- Gene ID
- 673
- UniProt ID
- P15056
- Sequence Similarities
- Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity.
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
- Protein Refseq
- NP_004324.2
- Function
- Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126).
May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
cAMP Signaling Pathway
MAPK Signaling Pathway
Throid Cancer
Cushing Syndrome
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BRAF"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-F(E) | Recombinant Anti-human BRAF Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| MHH-122-F(E) | Recombinant Human Anti-human BRAF Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-S(P) | Recombinant Anti-human BRAF Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-122-S(P) | Recombinant Human Anti-human BRAF Antibody scFv Fragment | WB, Neut, FuncS | scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1284CL | Anti-Human BRAF Recombinant Antibody (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-S(P) | Anti-Human BRAF Recombinant Antibody scFv Fragment (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-F(E) | Anti-Human BRAF Recombinant Antibody Fab Fragment (CCTCC C2012122) | IB, IF |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0379 | Hi-Affi™ Rabbit Anti-BRAF Recombinant Antibody (clone DS379AB) | WB, IHC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0148-CN | Rabbit Anti-BRAF Recombinant Antibody (clone CBACN-057) | WB, IHC, IP, FC | Rabbit IgG |
| MRO-0189-CN | Mouse Anti-BRAF Recombinant Antibody (clone F1-F4-E10) | WB, IHC, IF | Mouse IgG2b |
| ZG-0571J | Rabbit Anti-BRAF Recombinant Antibody (ZG-0571J) | ELISA, WB, IF | Rabbit IgG |
| VS3-QX130 | Mouse Anti-BRAF Recombinant Antibody (clone 4E1-E1-F9) | WB | Mouse IgG2a |
| VS3-QX131 | Mouse Anti-BRAF Recombinant Antibody (clone 1F1-F7-A8) | WB, ICC | Mouse IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0457 | PE-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0458 | APC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0459 | FITC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-YF341 | B*27:05/Human BRAF (600E) (GRFGLATEK) MHC Pentamer | FCM | |
| MHC-YF342 | B*27:05/Human BRAF (600V) (GRFGLATVK) MHC Pentamer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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