Recombinant Rabbit Anti-SNCA Antibody (clone R01-4E1) (CAT#: VS3-FY1367)

• Immunogen
• Recombinant protein of human α-synuclein.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Rat SNCA

• Species Reactivity
• Human, Rat

• Clone
• R01-4E1

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 14 kDa; Observed MW: 18 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• Synuclein Alpha; Synuclein, Alpha (Non A4 Component Of Amyloid Precursor); Alpha-Synuclein; PARK1; NACP; Parkinson Disease (Autosomal Dominant, Lewy Body) 4; Non A4 Component Of Amyloid Precursor; Non-A4 Component Of Amyloid Precursor

• Gene ID
• 6622

• UniProt ID
• P37840

• Sequence Similarities
• Belongs to the synuclein family.

• Cellular Localization
• Amyloid, Cell projection, Cytoplasm, Membrane, Nucleus, Secreted, Synapse

• Post Translation Modifications
• Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
  Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
  Ubiquitinated. The predominant conjugate is the diubiquitinated form.
  Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.

• Protein Refseq
• NP_000336.1; NP_001139526.1; NP_001139527.1

• Function
• Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:28288128, PubMed:30404828, PubMed:20798282, PubMed:26442590).
  Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828).
  Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828).
  Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282).
  This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282).
  Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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