Recombinant Rabbit Anti-TP53 Antibody (clone R06-7C9)
CAT#: VS3-FY1473
This product is a recombinant rabbit antibody that recognizes TP53. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R06-7C9 is specific for human, mouse, rat TP53. The antigen is synthetic acetyl peptide corresponding to residues surrounding lys370 of human p53.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg
Normal Tissue
Figure 5 Oral mucosa
Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg
Normal Tissue
Figure 6 Colon
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53
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WB
Figure 1. Recombinant Rabbit Anti-TP53 Antibody (clone R06-7C9) in WB.
Western Blot analysis of Acetyl-p53 (Lys370) in rat brain lysates using Acetyl-p53 (Lys370) Antibody.
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Specifications
- Immunogen
- Synthetic acetyl peptide corresponding to residues surrounding Lys370 of human p53.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat TP53
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-7C9
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 44 kDa; Observed MW: 53 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Tumor Protein P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Cellular Tumor Antigen P53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome
- Gene ID
- 7157
- UniProt ID
- P04637
- Cellular Localization
- Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Mitochondrion, Nucleus
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-072 | Recombinant Anti-p53 VHH Single Domain Antibody (PNBL-072) | Inhib, IP, WB, ELISA, FuncS | Llama VHH |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1423CT | Recombinant Mouse anti-Human TP53 Monoclonal antibody (5E2) | ELISA, FC, ICC, IF, IHC-Fr, IHC-P, IP, WB | |
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| MRO-2351-CN | Rabbit Anti-TP53 Recombinant Antibody (clone CBACN-640) | WB | Rabbit IgG |
| MRO-2352-CN | Rabbit Anti-TP53 Recombinant Antibody (clone CBACN-641) | WB | Rabbit IgG |
| ZG-0422U | Rabbit Anti-TP53 Recombinant Antibody (clone H21-E), FITC | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3644 | Rabbit Anti-TP53 Recombinant Antibody (clone DS3644AB) | IHC-P | Rabbit IgG |
| MOR-4524 | Rabbit Anti-TP53 Recombinant Antibody (clone TH32DS) | WB, IF, ICC | Rabbit IgG |
| MOR-4698 | Rabbit Anti-TP53 Recombinant Antibody (clone TH212DS) | WB, IF, ICC, FC, ChIP, FRET | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MD-ZBF-YC001 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-1.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC002 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-2.1) | WB, ICC, IHC | Mouse antibody |
| MD-ZBF-YC003 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-3.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC004 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-4.1) | WB, ICC | Mouse antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); Fab Fragment | IHC, WB, ELISA | Mouse Fab |
| HPAB-0002-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 240); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0003-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 246); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0004-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 421); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0662-YJ-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone Ab421); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0633CQ | Mouse Anti-TP53 Recombinant Antibody (clone p53-11) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY261 | AbPlus™ Anti-TP53 Magnetic Beads (240) | IP, Protein Purification | |
| VS-0724-YC1290 | AbPlus™ Anti-TP53 Magnetic Beads (VS-0724-YC1290) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY361 | Mouse Anti-NHP TP53 Recombinant Antibody (clone BP53-12) | WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC186 | Recombinant Anti-TP53 (AA 213-218 x AA 370-378) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
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