Recombinant Rabbit Anti-TP53 Antibody (clone R07-6A7) (CAT#: VS3-FY1474)

This product is a recombinant rabbit antibody that recognizes TP53. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R07-6A7 is specific for human TP53. The antigen is human p53 recombinant protein.

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WB

Figure 1. Recombinant Rabbit Anti-TP53 Antibody (clone R07-6A7) in WB.

Figure 1. Recombinant Rabbit Anti-TP53 Antibody (clone R07-6A7) in WB.

Western Blot analysis of p53 in Jurkat lysates using p53 antibody.

ICC

Figure 2. Recombinant Rabbit Anti-TP53 Antibody (clone R07-6A7) in ICC.

Figure 2. Recombinant Rabbit Anti-TP53 Antibody (clone R07-6A7) in ICC.

Immunocytochemical analysis of p53 (green) in MCF-7 using p53 antibody and DAPI (blue).


Specifications

  • Immunogen
  • human p53 recombinant protein.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human TP53
  • Species Reactivity
  • Human
  • Clone
  • R07-6A7
  • Applications
  • Western Blot, Immunocytochemistry, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 44 kDa; Observed MW: 53 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Tumor Protein P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Cellular Tumor Antigen P53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome
  • Cellular Localization
  • Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Mitochondrion, Nucleus

Recommended Products

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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