Is this antibody validated for Surface Plasmon Resonance?

Yes, this Recombinant Human Antibody has been tested in Surface Plasmon Resonance (SPR), as well as ELISA, Flow Cytometry, Western Blot, Inhibition, and Immunohistochemistry. It binds to amino acids 1-134 of human Axl.

Does this antibody block ligand binding?

Yes, this antibody is suitable for Blocking and Inhibition assays. It targets AXL, a receptor tyrosine kinase that binds Gas6, and can be used to study signal transduction pathways involving cell growth and migration.

Human Anti-AXL Recombinant Antibody (HPAB-0600-CN)

CAT#: HPAB-0600-CN

This product is a recombinant human antibody that recognizes AXL. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. The antibody has been tested in some applications including Surface plasmon resonance (SPR), ELISA, Flow cytometry (FC), Western blot (WB), Inhibition, Immunohistochemistry (IHC), Block.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, actin filaments & vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75217/1899_C1_1_selected.jpg

Normal Tissue Figure 2 IHC staining of human thyroid gland

Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32501/ihc_selected.jpg

Normal Tissue Figure 3 Skeletal muscle

Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_B_1_6.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_8_3.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_9_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_4_6.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (fHDF/TERT166, U-251 MG)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167601-AXL

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Specifications

Host Species
Human

Derivation
A Fab phage display library

Type
Human IgG

Specificity
Human AXL

Species Reactivity
Human, Mouse

Applications
SPR, ELISA, FC, WB, Inhib, FuncS, IHC, BL

Related Disease
Tumor

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
ARK; UFO; JTK11; Tyro7

Gene ID
558

UniProt ID
P30530

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Breast cancer biomarkers at key points during disease progression

Validated For SPR

We utilized this antibody in Surface Plasmon Resonance experiments and it showed excellent binding kinetics. It binds the extracellular domain of AXL. It also worked well in blocking assays to inhibit Gas6 binding.

Versatile AXL Antibody

This is one of the most versatile antibodies we have; we used it for Flow Cytometry, WB, and IHC. The results were consistent across all applications. It is essential for our research on tumor migration and aggregation.

Q&As

Is this antibody validated for Surface Plasmon Resonance?

A: Yes, this Recombinant Human Antibody has been tested in Surface Plasmon Resonance (SPR), as well as ELISA, Flow Cytometry, Western Blot, Inhibition, and Immunohistochemistry. It binds to amino acids 1-134 of human Axl.
Does this antibody block ligand binding?

A: Yes, this antibody is suitable for Blocking and Inhibition assays. It targets AXL, a receptor tyrosine kinase that binds Gas6, and can be used to study signal transduction pathways involving cell growth and migration.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-0600-CN, RRID: AB_3713838)

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0600-CN.pdf

MSDS

MSDS - HPAB-0600-CN.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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