Human Anti-AXL (clone Enapotamab) scFv-Fc Chimera
CAT#: VS-0425-FY66
AXL is a receptor tyrosine kinase involved in cell survival and migration, and its targeting by this scFv-Fc fusion protein shows promise in cancer therapy, particularly for cancers like lung and pancreatic cancer that often express high levels of AXL.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, actin filaments & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75217/1899_C1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32501/ihc_selected.jpg
Normal Tissue
Figure 3 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_B_1_6.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (fHDF/TERT166, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167601-AXL
❮
❯
Specifications
- Immunogen
- Recombinant human AXL protein as an immunogen.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human AXL
- Species Reactivity
- Human
- Clone
- Enapotamab
- Applications
- ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/5000-1/10000
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- AXL Receptor Tyrosine Kinase; AXL Oncogene; EC 2.7.10.1; UFO; Tyrosine-Protein Kinase Receptor UFO; AXL Transforming Sequence/Gene
- Gene ID
- 558
- UniProt ID
- P30530
- Long Name
- AXL Receptor Tyrosine Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Monoubiquitinated upon GAS6-binding.
A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
Glycosylation at Asn43, Asn157, Asn198, Asn339, Asn345, and Asn401
Ubiquitination at Lys540
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect UFO_HUMAN (1983)
Glycosylation from GlyGen 9 sites, 13 N-linked glycans (4 sites), 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001265528.1; NP_001690.2; NP_068713.2
- Function
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation.
Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL.
Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11.
Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2.
Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase.
GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0425-FY66. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone Enapotamab"
- CAT
- Product Name
See other products for "AXL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0139CL | Anti-Human AXL Recombinant Antibody (hzl613F12-24 DAR2) | ELISA, IF, FC, Inhib, ICFC | Humanized Antibody |
| TAB-0139CL-S(P) | Anti-Human AXL Recombinant Antibody scFv Fragment (hzl613F12-24 DAR2) | ELISA, IF, FC, Inhib, ICFC | Humanized Antibody |
| TAB-0140CL-S(P) | Anti-Human AXL Recombinant Antibody scFv Fragment (hzl613F12-33 DAR4) | ELISA, IF, FC, Inhib, ICFC | Humanized Antibody |
| TAB-0139CL-F(E) | Anti-Human AXL Recombinant Antibody Fab Fragment (hzl613F12-24 DAR2) | ELISA, IF, FC, Inhib, ICFC | Humanized Antibody |
| TAB-0140CL-F(E) | Anti-Human AXL Recombinant Antibody Fab Fragment (hzl613F12-33 DAR4) | ELISA, IF, FC, Inhib, ICFC | Humanized Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0335CL-S(P) | Anti-Human AXL Recombinant Antibody scFv Fragment (Ax225 (was demonstrated to recognize FND1 of AXL)) | WB, FuncS, ELISA | |
| TAB-0336CL-S(P) | Anti-Human AXL Recombinant Antibody scFv Fragment | WB, FuncS, ELISA | |
| TAB-0138CL-F(E) | Mouse Anti-AXL Recombinant Antibody Fab Fragment (TAB-0138CL-S(P)) | ELISA, IF, FC, Inhib, ICFC | |
| TAB-0188CL-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (TAB-0188CL-F(E)) | WB, FuncS, ELISA, FC | Mouse Fab |
| TAB-1087CL-F(E) | Human Anti-AXL Recombinant Antibody; Fab Fragment (TAB-1087CL-F(E)) | ELISA, FuncS | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0189CL-F(E) | Human Anti-AXL Recombinant Antibody; Fab Fragment (TAB-0189CL-F(E)) | WB, FuncS, ELISA, FC | Chimeric (Mouse/Human) Fab |
| TAB-1090CL-F(E) | Human Anti-AXL Recombinant Antibody; Fab Fragment (TAB-1090CL-F(E)) | ELISA, FuncS | Chimeric (mouse/human) Fab |
| TAB-1091CL-F(E) | Human Anti-AXL Recombinant Antibody; Fab Fragment (TAB-1091CL-F(E)) | ELISA, FuncS | Chimeric (mouse/human) Fab |
| TAB-1092CL-F(E) | Human Anti-AXL Recombinant Antibody; Fab Fragment (TAB-1092CL-F(E)) | ELISA, FuncS | Chimeric (mouse/human) Fab |
| TAB-1071CLV-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (TAB-1071CLV-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3970 | Hi-Affi™ Rabbit Anti-AXL Recombinant Antibody (clone SI30DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1616LY | Human Anti-AXL Recombinant Antibody (HPAB-1616LY) | ELISA | Humanized IgG |
| HPAB-1617LY | Human Anti-AXL Recombinant Antibody (HPAB-1617LY) | ELISA | Humanized IgG |
| HPAB-1618LY | Human Anti-AXL Recombinant Antibody (HPAB-1618LY) | ELISA | Humanized IgG |
| HPAB-1619LY | Human Anti-AXL Recombinant Antibody (HPAB-1619LY) | ELISA | Humanized IgG |
| HPAB-1850LY | Mouse Anti-AXL Recombinant Antibody (HPAB-1850LY) | ELISA, IA, FC, IHC, WB, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0416-YC-S(P) | Human Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-M0416-YC-S(P)) | ELISA | Human scFv |
| HPAB-1850LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1850LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| HPAB-1851LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1851LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| HPAB-1852LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1852LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| HPAB-1853LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1853LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1850LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1850LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1851LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1851LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1852LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1852LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1853LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1853LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1854LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1854LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY26 | AbPlus™ Anti-AXL Magnetic Beads (VS-0424-XY26) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY44 | Rabbit Anti-NHP AXL Recombinant Antibody (VS-1024-XY44) | WB, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY2 | CytoStream™ Rat Anti-AXL Recombinant Antibody (clone 175128) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC363 | Recombinant Anti-AXL Vesicular Antibody, EV Displayed (VS-0425-YC363) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC20 | Recombinant Anti-AXL (AA 1-134 x AA 222-234) Biparatopic Antibody, Tandem scFv | Block, ELISA, FC, IHC, SPR, WB | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
-4.jpg)
Application: FC, FuncS, IA, IF, IP, IHC
Application: ELISA, WB, Microarray, Block
-2.png)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
