Human Anti-AXL (clone Enapotamab) scFv-Fc Chimera (CAT#: VS-0425-FY66)
AXL is a receptor tyrosine kinase involved in cell survival and migration, and its targeting by this scFv-Fc fusion protein shows promise in cancer therapy, particularly for cancers like lung and pancreatic cancer that often express high levels of AXL.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, actin filaments & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75217/1899_C1_1_selected.jpg

(Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32501/ihc_selected.jpg

(Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_B_1_6.jpg

(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_8_3.jpg

(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_9_5.jpg

(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_4_6.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167601-AXL
Specifications
- Immunogen
- Recombinant human AXL protein as an immunogen.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human AXL
- Species Reactivity
- Human
- Clone
- Enapotamab
- Applications
- ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/5000-1/10000
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- AXL Receptor Tyrosine Kinase; AXL Oncogene; EC 2.7.10.1; UFO; Tyrosine-Protein Kinase Receptor UFO; AXL Transforming Sequence/Gene
- Gene ID
- 558
- UniProt ID
- P30530
- Long Name
- AXL Receptor Tyrosine Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Monoubiquitinated upon GAS6-binding.
A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
Glycosylation at Asn43, Asn157, Asn198, Asn339, Asn345, and Asn401
Ubiquitination at Lys540
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect UFO_HUMAN (1983)
Glycosylation from GlyGen 9 sites, 13 N-linked glycans (4 sites), 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001265528.1; NP_001690.2; NP_068713.2
- Function
- Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation.
Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL.
Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11.
Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2.
Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase.
GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone Enapotamab"
See other products for "AXL"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0138CL | Mouse Anti-AXL Recombinant Antibody (TAB-0138CL) | ELISA, IF, FC, Inhib, ICFC | |
TAB-0188CL | Mouse Anti-AXL Recombinant Antibody (TAB-0188CL) | ELISA | Mouse IgG |
TAB-1087CL | Human Anti-AXL Recombinant Antibody (TAB-1087CL) | ADCC | Chimeric (mouse/human) IgG1, κ |
TAB-0335CL-S(P) | Anti-Human AXL Recombinant Antibody scFv Fragment (Ax225 (was demonstrated to recognize FND1 of AXL)) | WB, FuncS, ELISA | |
TAB-0336CL-S(P) | Anti-Human AXL Recombinant Antibody scFv Fragment | WB, FuncS, ELISA |
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