Anti-EDB Antibody-Cytokine Fusion Protein, scFv (L19)-TNFα
CAT#: ACFP-SH092
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EDB scFv to TNFα. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the EDB as well as the biological activity of TNFα. The fusion protein displays a potent antitumor activity in several immunocompetent murine models of cancer but do not lead to complete remissions of established aggressive tumors. The observed toxicity profile was mild and reversible when intravenously administered to patients with advanced progressive solid tumors in an outpatient setting. This immunocytokine was designed for treating Advanced solid tumors, Advanced pancreatic cancer, Metastatic melanoma.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_4.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_5_6.jpg
Normal Tissue
Figure 5 Placenta
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC, hTERT-HME1, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115414-FN1
❮
❯
Specifications
- Target
- EDB
- Molecule Class
- scFv-TNFα
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- L19
- Related Disease
- Advanced solid tumors, Advanced pancreatic cancer, Metastatic melanoma
- Cytokine 1
- TNFα
- Cytokines 1 Description
- Tumor necrosis factor (TNF, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD). While still controversial, studies of depression and IBD are currently being linked to TNF levels. Recombinant TNF is used as an immunostimulant under the INN tasonermin. TNF can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ACFP-SH092. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone L19"
- CAT
- Product Name
See other products for "FN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-193 | Anti-Human Fibronectin Extra Domain-B Recombinant Antibody (Radretumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | [(scFv - heavy - kappa) - IGHE - CH4]2 |
| TAB-001CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (MOR03255) | ELISA, BL, IHC | Human antibody |
| TAB-002CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (ME-4C) | ELISA, IHC | Human antibody |
| TAB-003CQ-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (1C5) | ELISA, WB | Human antibody |
| TAB-004CQ-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (TAB-004CQ-F(E)) | ELISA, IHC, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-852CQ | Mouse Anti-FN1 Recombinant Antibody (clone HFN7.1) | ELISA, WB, IP, Block | Mouse IgG1 |
| NEUT-853CQ | Mouse Anti-FN1 Recombinant Antibody (clone IST-9) | WB, RIA, ELISA, IHC-Fr, ICC, IF, Block, IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1326 | Hi-Affi™ Rabbit Anti-FN1 Recombinant Antibody (clone DS1326AB) | IHC-Fr, IHC-P, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0193-CN-S(P) | Mouse Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0193-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0194-CN-S(P) | Mouse Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0194-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0195-CN-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0195-CN-S(P)) | ELISA, FC, IHC | Human scFv |
| HPAB-S0037-YC-S(P) | Human Anti-FN1 Recombinant Antibody (clone CGS-2); scFv Fragment | ELISA, IB, IHC | Human scFv |
| HPAB-S0038-YC-S(P) | Human Anti-FN1 Recombinant Antibody (clone L19); scFv Fragment | ELISA, IF, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1110-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1110-FY-S(P)) | IHC | Human scFv |
| HPAB-1111-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1111-FY-S(P)) | IHC | Human scFv |
| HPAB-1112-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1112-FY-S(P)) | IHC | Human scFv |
| HPAB-1113-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1113-FY-S(P)) | IHC | Human scFv |
| HPAB-1114-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1114-FY-S(P)) | IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0037-YC | Human Anti-FN1 Recombinant Antibody (clone CGS-2) | ELISA, IB, IHC | Human IgG |
| HPAB-S0038-YC | Human Anti-FN1 Recombinant Antibody (clone L19) | ELISA, IF, IHC | Human IgG |
| ZG-0661F | Rabbit Anti-FN1 Recombinant Antibody (ZG-0661F) | IF, IHC-P, ELISA, RIA | Rabbit IgG |
| ZG-0662F | Rabbit Anti-FN1 Recombinant Antibody (ZG-0662F) | IF, IHC-P, ELISA, RIA | Rabbit IgG |
| ZG-0663F | Rabbit Anti-FN1 Recombinant Antibody (ZG-0663F) | IF, IHC-P, ELISA, RIA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NBD-0040-YJ-VHH | Camelid Anti-FN1 Recombinant Single Domain Antibody (NBD-0040-YJ-VHH) | ELISA, In vivo imaging | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY105 | AbPlus™ Anti-FN1 Magnetic Beads (HFN7.1) | IP, Protein Purification | |
| VS-0724-YC853 | AbPlus™ Anti-FN1 Magnetic Beads (VS-0724-YC853) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY204 | Mouse Anti-NHP FN1 Recombinant Antibody (clone C6F10) | WB, IHC, IF, IP | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY108 | Human Anti-FN1 (clone L19) scFv-Fc Chimera | IHC, ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2593 | Anti-FN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2594 | Anti-Rat FN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2595 | Anti-Mouse FN1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC87 | Recombinant Anti-FN1 ED-B (N-terminus x C-terminus) Biparatopic Antibody, Tandem scFv (Clone CGS-2 x Clone CGS-1) | ELISA | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC121 | Recombinant Anti-FN1 Eliminating Antibody, pH-Sensitive (VS-0625-YC121) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IF, WB
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: FC, IA, IF, IP, IHC, FuncS
Application: ELISA, FC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
