Can this antibody inhibit IL-6 activity?

Yes, the Recombinant Humanized Antibody BA399 is capable of binding to human IL-6 and inhibiting its activity. It is validated for Inhibition (Inhib) studies, as well as ELISA and Flow Cytometry (FC).

What type of antibody is this?

This is a Humanized monoclonal antibody of the IgG isotype. It is produced in CHO cells as a recombinant antibody. It binds specifically to IL-6 (Interleukin 6) and is suitable for research into inflammation and autoimmune diseases.

Anti-human IL6 Recombinant Antibody (BA399) (TAB-678LC)

CAT#: TAB-678LC

Recombinant monoclonal antibody BA399 is a humanized monoclonal antibody that specifically binds to human IL-6 and can be potentially used in the treatment of cachexia, rheumatoid arthritis and osteoporosis.

Published Data Gene Expression

ELISA

Figure 1 Competition ELISA analysis of BA001 and Anti-IL-6 Antibodies.

Figure 2 Normalized specific activity of the top 10 hits from BA399 affinity maturation by combinatorial protein synthesis (CPS).

The relative specific activity for each clone is normalized to that of Anti-IL-6 Ab BA399, shown as a horizontal black line.

Inhib

Figure 3 Inhibition of IL-6/sIL6R induced Stat3 phosphorylation in THP-1 cells by increasing anti-IL-6 Mab concentration.

The data are normalized based on Western blot for total Stat3.

Inhib

Figure 4 Inhibition of Stat3 phosphorylation in THP-1 cells stimulated by IL-6/sIL-6R by various anti-IL6 Mabs including 003, BA399, BA399-2 and BA399-9 using 100 ng/mL IL-6.

Inhib

Figure 5 Inhibition of serum amyloid A protein production by HepG2 cells stimulated with human IL-6, sIL-6R and IL-1b, by serial dilutions of anti-IL-6 Mabs CNTO136, BA399, BA399-2, BA399-9 at 24 hours.

Inhib

Figure 6 Inhibition of IL-6 induced DS-1 cell proliferation at 48 hours by anti-IL-6 Mabs BA001, BA399, BA436, BA802, BA808, BA840, BA848 and BA890.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg

Normal Tissue Figure 4 Urinary bladder

Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg

Normal Tissue Figure 5 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6

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Specifications

Host Species
Human

Derivation
Humanized

Type
Humanized antibody

Specificity
Human

Clone
BA399

Applications
ELISA, FC, Inhib

Related Disease
Cachexia, rheumatoid arthritis and osteoporosis

Applications

Application Notes
This antibody has been tested for use in Enzyme-Linked Immunosorbent Assay and Inhibition.

Target

Alternative Names
IL6; interleukin 6; HGF; HSF; BSF2; IL-6; IFNB2; interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor

Gene ID
3569

UniProt ID
B4DVM1

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Breast cancer biomarkers at key points during disease progression

Potent IL-6 Inhibition

We used the antibody BA399 for inhibition assays of IL-6. It effectively neutralized the cytokine's activity in our cell culture system. This Recombinant Humanized Antibody is highly specific to human IL-6 and is a valuable tool for studying inflammation.

Validated for ELISA and FC

In addition to inhibition, we used this antibody for ELISA and Flow Cytometry (FC). It performed well in both applications. The antibody is produced in CHO cells and is of IgG isotype. Storing it at -20°C keeps it stable for our long-term experiments.

Q&As

Can this antibody inhibit IL-6 activity?

A: Yes, the Recombinant Humanized Antibody BA399 is capable of binding to human IL-6 and inhibiting its activity. It is validated for Inhibition (Inhib) studies, as well as ELISA and Flow Cytometry (FC).
What type of antibody is this?

A: This is a Humanized monoclonal antibody of the IgG isotype. It is produced in CHO cells as a recombinant antibody. It binds specifically to IL-6 (Interleukin 6) and is suitable for research into inflammation and autoimmune diseases.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Inflammatory Bowel Diseases

Rheumatoid Arthritis

Graft-versus-Host Disease

AGE-RAGE Signaling Pathway in Diabetic Complications

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-678LC.pdf

MSDS

MSDS - TAB-678LC.pdf

COA

Certificate of Analysis Lookup

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