Anti-Human MUC1 Recombinant Antibody (huDMB5F3) (CAT#: TAB-425MZ)
Provided here is a humanized antibody that recognizes human MUC-1. This antibody internalization from the cell surface occurs in an efficient temperature-dependent manner. It also exerts specific cytotoxicity against cancer cells.
Specific Inquiry
ELISA
Figure 1 Immunoreactivity of DMB mAbs with MUC1-Xex.
ELISA plates were coated with 1 of 3 recombinant bacterial MUC1 proteins extracellular domain of the MUC1-X protein (dotted lines), MUC1 SEA module (dashed lines), or the uncleaved MUC1 4G-SEA module protein (dashed and dotted lines). The indicated mAbs were applied and bound antibody was detected as described in Materials and Methods.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
FuncS
Figure 2 Titration analysis of DMB monoclonal anti-MUC1 SEA module α/β junction antibodies to human breast cancer cells expressing MUC1 and affinity analysis.
Antibody DMB5F3 was shown to have the highest affinity.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
FC
Figure 3 Flow cytometry analyses were carried out with the chDMB5F3 antibody at the indicated concentrations using MUC1-negative parental DA3 cells (DA3-PAR) and mouse cell transfectants (DA3-TM) expressing human MUC1-TM (E and F, respectively).
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
ICC
Figure 4 MUC1+ cells were incubated with DyLight649-labeled DMB5F3 mAb at 4°C and at 37°C (G and H, respectively) and photographed by confocal laser microscopy.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
FuncS
Figure 5 Specificity and affinity of chDMB5F3 immunotoxin (IT) for cell killing of cancer cells.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
Specifications
- Host Species
- Human
- Type
- Humanized antibody
- Specificity
- Human
- Clone
- huDMB5F3
- Applications
- ELISA, FC, FuncS, ICC
- Related Disease
- Pancreatic cancer, breast cancer
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytotoxicityometry, Functional Assay, Immunocytochemistry.
Target
- Alternative Names
- MUC1; mucin 1, cell surface associated; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant;
- Gene ID
- 4582
- UniProt ID
- P15941
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2049-VHH | Recombinant Anti-human MUC1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| HPAB-AP504-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (HPAB-AP504-YC) | FC | Camel VHH |
| HPAB-AP505-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (HPAB-AP505-YC) | ELISA | Camel VHH |
| HPAB-0736-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0736-YJ-VHH) | ELISA | Camelid VHH |
| HPAB-0737-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (HPAB-0737-YJ-VHH) | ELISA | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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