Anti-Human MUC1 Recombinant Antibody (huDMB5F3) (CAT#: TAB-425MZ)

Provided here is a humanized antibody that recognizes human MUC-1. This antibody internalization from the cell surface occurs in an efficient temperature-dependent manner. It also exerts specific cytotoxicity against cancer cells.


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ELISA

Figure 1 Immunoreactivity of DMB mAbs with MUC1-Xex.

Figure 1 Immunoreactivity of DMB mAbs with MUC1-Xex.

ELISA plates were coated with 1 of 3 recombinant bacterial MUC1 proteins extracellular domain of the MUC1-X protein (dotted lines), MUC1 SEA module (dashed lines), or the uncleaved MUC1 4G-SEA module protein (dashed and dotted lines). The indicated mAbs were applied and bound antibody was detected as described in Materials and Methods.

Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.

FuncS

Figure 2 Titration analysis of DMB monoclonal anti-MUC1 SEA module α/β junction antibodies to human breast cancer cells expressing MUC1 and affinity analysis.

Figure 2 Titration analysis of DMB monoclonal anti-MUC1 SEA module α/β junction antibodies to human breast cancer cells expressing MUC1 and affinity analysis.

Antibody DMB5F3 was shown to have the highest affinity.

Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.

FC

Figure 3 Flow cytometry analyses were carried out with the chDMB5F3 antibody at the indicated concentrations using MUC1-negative parental DA3 cells (DA3-PAR) and mouse cell transfectants (DA3-TM) expressing human MUC1-TM (E and F, respectively).

Figure 3 Flow cytometry analyses were carried out with the chDMB5F3 antibody at the indicated concentrations using MUC1-negative parental DA3 cells (DA3-PAR) and mouse cell transfectants (DA3-TM) expressing human MUC1-TM (E and F, respectively).

Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.

ICC

Figure 4 MUC1+ cells were incubated with DyLight649-labeled DMB5F3 mAb at 4°C and at 37°C (G and H, respectively) and photographed by confocal laser microscopy.

Figure 4 MUC1+ cells were incubated with DyLight649-labeled DMB5F3 mAb at 4°C and at 37°C (G and H, respectively) and photographed by confocal laser microscopy.

Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.

FuncS

Figure 5 Specificity and affinity of chDMB5F3 immunotoxin (IT) for cell killing of cancer cells.

Figure 5 Specificity and affinity of chDMB5F3 immunotoxin (IT) for cell killing of cancer cells.

Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.


Specifications

  • Host Species
  • Human
  • Type
  • Humanized antibody
  • Specificity
  • Human
  • Clone
  • huDMB5F3
  • Applications
  • ELISA, FC, FuncS, ICC
  • Related Disease
  • Pancreatic cancer, breast cancer

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytotoxicityometry, Functional Assay, Immunocytochemistry.

Target

  • Alternative Names
  • MUC1; mucin 1, cell surface associated; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone huDMB5F3"

See other products for "MUC1"

Humanized Antibody

CAT Product Name Application Type
TAB-173 Anti-Human MUC1 Recombinant Antibody (Cantuzumab Ravtansine) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1 - kappa
TAB-166 Humanized Anti-MUC1 Recombinant Antibody (clone Cantuzumab) IP, IF, FuncS, FC, Neut, ELISA, ICC Humanized (from mouse) IgG1, κ
TAB-037-F(E) Anti-Human MUC1 Recombinant Antibody Fab Fragment (Clivatuzumab Tetraxetan) Neut, ELISA, IF, IP, FuncS, FC, IHC Fab - G1 - kappa
TAB-413MZ-F(E) Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-413MZ-F(E)) ELISA Humanized Fab
TAB-425MZ-F(E) Anti-Human MUC1 Recombinant Antibody Fab Fragment (huDMB5F3) FC Humanized antibody

Immunotoxin

CAT Product Name Application Type
AGTO-L036E anti-MUC1 immunotoxin C242 (Fab)-PE Cytotoxicity assay, Functional assay
AGTO-L064E anti-MUC1 immunotoxin H23 (scFv)-PE Cytotoxicity assay, Functional assay

Fab Glycosylation

CAT Product Name Application Type
Gly-012LC Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) ELISA, FC, IHC Humanized antibody
Gly-120LC Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) ELISA Mouse antibody

Sialylated IgG Glycan

CAT Product Name Application Type
Gly-012LC-1 Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) ELISA, FC, IHC Humanized antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-109LC Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) ELISA Human antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0379MZ Chicken Anti-MUC1 Polyclonal IgY IHC, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2320 Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) IHC-P, IHC-Fr IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H56 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Pemtumomab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-H65 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Sontuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody
AFC-TAB-026ML Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Gatipotuzumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-166 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Cantuzumab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody
AFC-TAB-H77 Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) ELISA, FC, IP, FuncS, IF IgG1, κ

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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