What immunogen was used for this antibody?

The antibody (clone CV70) was generated using the Waldenstroms macroglobulineamia cell line WM-1 as the immunogen. It specifically reacts with Human CD70 and is validated for several applications including IP and IHC-Fr.

What is the clone name of this antibody?

The clone name for this Recombinant Mouse Anti-Human CD70 Antibody is CV70. It is an IgG1 isotype expressed in CHO cells and is suitable for ELISA, Flow Cytometry, Functional Studies, and more.

Recombinant Mouse Anti-Human CD70 Antibody (clone CV70)

CAT#: MOB-1639MZ

This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human CD70.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line SiHa

Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46582/1486_B2_3_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46582/1559_D6_2_red_green.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (SiHa, THP-1, U-251 MG, U-266/70, U-266/84, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000125726-CD70

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Specifications

Immunogen
Waldenstroms macroglobulineamia cell line WM-1

Host Species
Mouse

Type
Mouse antibody

Specificity
Human CD70

Species Reactivity
Human

Clone
CV70

Applications
ELISA, FC, FuncS, IHC-Fr, IP

Target

Alternative Names
CD70; Human CD70

Gene ID
970

UniProt ID
P32970

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Breast cancer biomarkers at key points during disease progression

Successful Immunoprecipitation

We used clone CV70 for immunoprecipitating CD70 from cell lysates. It pulled down the target protein effectively, which we then confirmed via Western blot. The antibody is very specific to human CD70 and works well for functional studies.

Worked For Immunohistochemistry

Our lab tested this recombinant antibody on frozen tissue sections. The immunohistochemistry results were positive, showing distinct staining patterns relevant to CD70 expression. It is a robust reagent for both flow cytometry and tissue analysis.

Q&As

What immunogen was used for this antibody?

A: The antibody (clone CV70) was generated using the Waldenstroms macroglobulineamia cell line WM-1 as the immunogen. It specifically reacts with Human CD70 and is validated for several applications including IP and IHC-Fr.
What is the clone name of this antibody?

A: The clone name for this Recombinant Mouse Anti-Human CD70 Antibody is CV70. It is an IgG1 isotype expressed in CHO cells and is suitable for ELISA, Flow Cytometry, Functional Studies, and more.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-1639MZ.pdf

MSDS

MSDS - MOB-1639MZ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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