Rabbit Anti-F3 Recombinant Antibody (VS3-CJ640)
CAT#: VS3-CJ640
This product is a rabbit antibody that recognizes human, mouse, and rat F3.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49292/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69132/1343_C8_1_selected.jpg
Subcellular Location
Figure 3 IHC staining of human nasopharynx
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_B_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_2_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A-431, HaCaT, HBEC3-KT, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117525-F3
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat F3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- TF; TFA; CD142
- Gene ID
- 2152
- UniProt ID
- P13726
- Sequence Similarities
- Belongs to the tissue factor family.
- Cellular Localization
- Membrane, Secreted
- Protein Refseq
- NP_001171567.1; NP_001984.1
- Function
- Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-043 | Mouse Anti-F3 Recombinant Antibody (clone 5G9); scFv Fragment | Block | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-276CQ-S(P) | Human Anti-F3 Recombinant Antibody; scFv Fragment (TAB-276CQ-S(P)) | ELISA, FC | Humanized scFv |
| TAB-278CQ-S(P) | Human Anti-F3 Recombinant Antibody; scFv Fragment (TAB-278CQ-S(P)) | ELISA, FC | Humanized scFv |
| TAB-276CQ-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (TAB-276CQ-F(E)) | ELISA, FC | Humanized Fab |
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| TAB-277CQ-F(E) | Mouse Anti-F3 Recombinant Antibody; Fab Fragment (TAB-277CQ-F(E)) | ELISA, FC | Mouse Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0573CQ | Mouse Anti-F3 Recombinant Antibody (clone hATR-5) | ELISA, Inhib | Mouse IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0172CQ-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0172CQ-F(E)) | ELISA, FC | Chimeric (mouse/human) Fab |
| HPAB-0173CQ-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0173CQ-F(E)) | ELISA, FC | Chimeric (mouse/human) Fab |
| HPAB-0174CQ-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0174CQ-F(E)) | ELISA, FC | Chimeric (mouse/human) Fab |
| HPAB-0175CQ-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0175CQ-F(E)) | ELISA, WB, Inhib | Humanized Fab |
| HPAB-1000WJ-F(E) | Human Anti-F3 Recombinant Antibody (clone JT C5); Fab Fragment | ELISA, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-473CQ | Afuco™ Anti-F3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-473CQ) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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