Mouse Anti-FAP Recombinant Antibody (clone F19) (CAT#: HPAB-A1368-YJ)

This product is a Mouse antibody that recognizes FAP.

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  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IP

Figure 1 Immunoprecipitation of purified FAPmCD8 fusion protein followed by 8% SDS-polyacrylamide gel electrophoresis and dipeptidyl peptidase overlay assay.

Figure 1 Immunoprecipitation of purified FAPmCD8 fusion protein followed by 8% SDS-polyacrylamide gel electrophoresis and dipeptidyl peptidase overlay assay.

Park, J. E., Lenter, M. C., Zimmermann, R. N., Garin-Chesa, P., Old, L. J., & Rettig, W. J. (1999). Fibroblast activation protein, a dual specificity serine protease expressed in reactive human tumor stromal fibroblasts. Journal of Biological Chemistry, 274(51), 36505-36512.

IF

Figure 2 Immunofluorescence microscopy of 293FAPwt transfectants (A) and vector-transfected parental 293 cells (B) with antiFAP mAb F19 followed by fluorescein isothiocyanate-labeled goat antimouse IgG.

Figure 2 Immunofluorescence microscopy of 293FAPwt transfectants (A) and vector-transfected parental 293 cells (B) with antiFAP mAb F19 followed by fluorescein isothiocyanate-labeled goat antimouse IgG.

IHC

Figure 3 Immunohistochemical detection of FAP using mAb F19 in a colon carcinoma (A) or matched control normal colon tissue from the same patient (B).

Figure 3 Immunohistochemical detection of FAP using mAb F19 in a colon carcinoma (A) or matched control normal colon tissue from the same patient (B).

IA

Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.

Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.

Block

Figure 5 Epitope analysis of FAP-specific MAbs.

Figure 5 Epitope analysis of FAP-specific MAbs.

RIA

Figure 6 Analysis of FAPa cell surface expression on FB20 leptomeningeal fibroblasts treated with growth and differentiation factors for 24 hr.

Figure 6 Analysis of FAPa cell surface expression on FB20 leptomeningeal fibroblasts treated with growth and differentiation factors for 24 hr.

Indirect radiobinding assays were carried out with MAb F19 or negative control mouse IgG, followed by lEI-goat anti-mouse Ig.

Rettig, W. J., Su, S. L., Fortunato, S. R., Scanlan, M. J., Mohan Raj, B. K., Garin‐Chesa, P., ... & Old, L. J. (1994). Fibroblast activation protein: purification, epitope mapping and induction by growth factors. International journal of cancer, 58(3), 385-392.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human FAP
  • Species Reactivity
  • Human
  • Clone
  • F19
  • Applications
  • ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
  • Related Disease
  • Tumor

Product Property

  • Purification
  • Protein A/G Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the Certificate of Analysis for the lot-specific concentration.
  • Buffer
  • PBS
  • Storage
  • Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody can be used in Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoassay, Radioimmunoassay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, and Blocking. Optimal working dilutions should be determined experimentally by the investigator.

Target

  • Alternative Names
  • FAPA; SIMP; DPPIV; FAPalpha; prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; dipeptidyl peptidase FAP; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; seprase; surface-expressed protease

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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