Can this antibody handle high levels of stress during sample preparation?

While reasonably robust, avoid extreme pH, high temperatures, and harsh chemicals to maintain antibody integrity.

What are the benefits of using this antibody in a multiplex assay?

Allows simultaneous detection of multiple targets, saving time and sample material while providing comprehensive data.

Can I use this antibody with both fresh and formalin-fixed samples?

Yes, it is typically validated for both fresh and FFPE samples. However, always confirm with the datasheet or preliminary tests.

Mouse Anti-FAP Recombinant Antibody (clone F19)

CAT#: HPAB-A1368-YJ

This product is a Mouse antibody that recognizes FAP.

Published Data Tested Data Gene Expression

Figure 1 Immunoprecipitation of purified FAPmCD8 fusion protein followed by 8% SDS-polyacrylamide gel electrophoresis and dipeptidyl peptidase overlay assay.

Park, J. E., Lenter, M. C., Zimmermann, R. N., Garin-Chesa, P., Old, L. J., & Rettig, W. J. (1999). Fibroblast activation protein, a dual specificity serine protease expressed in reactive human tumor stromal fibroblasts. Journal of Biological Chemistry, 274(51), 36505-36512.

Figure 2 Immunofluorescence microscopy of 293FAPwt transfectants (A) and vector-transfected parental 293 cells (B) with antiFAP mAb F19 followed by fluorescein isothiocyanate-labeled goat antimouse IgG.

IHC

Figure 3 Immunohistochemical detection of FAP using mAb F19 in a colon carcinoma (A) or matched control normal colon tissue from the same patient (B).

$Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.$

Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.

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$Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.$

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Figure 1 Anti-FAP Antibody in WB.

Western blot analysis of HPAB-A1368-YJ was performed by loading Human FAP Protein.

ELISA

Figure 2 Anti-FAP Antibody in ELISA.

ELISA analysis of HPAB-A1368-YJ was performed by coating with Human FAP Protein.

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Normal Tissue Figure 1 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_9_3.jpg

Normal Tissue Figure 2 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_8_4.jpg

Normal Tissue Figure 3 Kidney

Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_7_5.jpg

Normal Tissue Figure 4 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_4_6.jpg

Normal Tissue Figure 5 Smooth muscle

Smooth muscle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_B_6_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ hTERT+, hTERT-RPE1, U-138 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000078098-FAP

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
Human FAP

Species Reactivity
Human

Clone
F19

Applications
ELISA, FuncS, RIA, IP, IF, IHC, IA, Block

Related Disease
Tumor

Product Property

Purification
Protein A/G Purified

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.

Buffer
PBS

Storage
Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
This antibody can be used in Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoassay, Radioimmunoassay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, and Blocking. Optimal working dilutions should be determined experimentally by the investigator.

Target

Alternative Names
FAPA; SIMP; DPPIV; FAPalpha; prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; dipeptidyl peptidase FAP; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; seprase; surface-expressed protease

Gene ID
2191

UniProt ID
Q12884

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Breast cancer biomarkers at key points during disease progression

Achieved High Sensitivity

Attained a good level of sensitivity in identifying proteins with low abundance, which was crucial for my investigation.

Worked with Automation

This antibody was utilized with automated staining equipment without any problems. incredibly practical.

Stable and Reliable

The antibody was found to be consistently stable and dependable throughout time, which is essential for reproducibility.

Q&As

Can this antibody handle high levels of stress during sample preparation?

A: While reasonably robust, avoid extreme pH, high temperatures, and harsh chemicals to maintain antibody integrity.
What are the benefits of using this antibody in a multiplex assay?

A: Allows simultaneous detection of multiple targets, saving time and sample material while providing comprehensive data.
Can I use this antibody with both fresh and formalin-fixed samples?

A: Yes, it is typically validated for both fresh and FFPE samples. However, always confirm with the datasheet or preliminary tests.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-A1368-YJ, RRID: AB_3111429)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-A1368-YJ.pdf

MSDS

MSDS - HPAB-A1368-YJ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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