Mouse Anti-FAP Recombinant Antibody (clone F19) (CAT#: HPAB-A1368-YJ)
This product is a Mouse antibody that recognizes FAP.
Specific Inquiry
IP
Figure 1 Immunoprecipitation of purified FAPmCD8 fusion protein followed by 8% SDS-polyacrylamide gel electrophoresis and dipeptidyl peptidase overlay assay.
Park, J. E., Lenter, M. C., Zimmermann, R. N., Garin-Chesa, P., Old, L. J., & Rettig, W. J. (1999). Fibroblast activation protein, a dual specificity serine protease expressed in reactive human tumor stromal fibroblasts. Journal of Biological Chemistry, 274(51), 36505-36512.
IF
Figure 2 Immunofluorescence microscopy of 293FAPwt transfectants (A) and vector-transfected parental 293 cells (B) with antiFAP mAb F19 followed by fluorescein isothiocyanate-labeled goat antimouse IgG.
IHC
Figure 3 Immunohistochemical detection of FAP using mAb F19 in a colon carcinoma (A) or matched control normal colon tissue from the same patient (B).
IA
Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.
Block
Figure 5 Epitope analysis of FAP-specific MAbs.
RIA
Figure 6 Analysis of FAPa cell surface expression on FB20 leptomeningeal fibroblasts treated with growth and differentiation factors for 24 hr.
Indirect radiobinding assays were carried out with MAb F19 or negative control mouse IgG, followed by lEI-goat anti-mouse Ig.
Rettig, W. J., Su, S. L., Fortunato, S. R., Scanlan, M. J., Mohan Raj, B. K., Garin‐Chesa, P., ... & Old, L. J. (1994). Fibroblast activation protein: purification, epitope mapping and induction by growth factors. International journal of cancer, 58(3), 385-392.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human FAP
- Species Reactivity
- Human
- Clone
- F19
- Applications
- ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
- Related Disease
- Tumor
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the Certificate of Analysis for the lot-specific concentration.
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody can be used in Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoassay, Radioimmunoassay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, and Blocking. Optimal working dilutions should be determined experimentally by the investigator.
Target
- Alternative Names
- FAPA; SIMP; DPPIV; FAPalpha; prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; dipeptidyl peptidase FAP; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; seprase; surface-expressed protease
- Gene ID
- 2191
- UniProt ID
- Q12884
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-211 | Anti-Human FAP Recombinant Antibody (Sibrotuzumab) | ELISA, IP, FC, FuncS, Neut, IF, WB | IgG1 - kappa |
Customer Reviews and Q&As
Customer Review
Q&As
-
Achieved High Sensitivity
Attained a good level of sensitivity in identifying proteins with low abundance, which was crucial for my investigation. -
Worked with Automation
This antibody was utilized with automated staining equipment without any problems. incredibly practical. -
Stable and Reliable
The antibody was found to be consistently stable and dependable throughout time, which is essential for reproducibility.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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