Mouse Anti-FAP Recombinant Antibody (clone F19) (CAT#: HPAB-A1368-YJ)
This product is a Mouse antibody that recognizes FAP.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Immunoprecipitation of purified FAPmCD8 fusion protein followed by 8% SDS-polyacrylamide gel electrophoresis and dipeptidyl peptidase overlay assay.
Park, J. E., Lenter, M. C., Zimmermann, R. N., Garin-Chesa, P., Old, L. J., & Rettig, W. J. (1999). Fibroblast activation protein, a dual specificity serine protease expressed in reactive human tumor stromal fibroblasts. Journal of Biological Chemistry, 274(51), 36505-36512.
Figure 2 Immunofluorescence microscopy of 293FAPwt transfectants (A) and vector-transfected parental 293 cells (B) with antiFAP mAb F19 followed by fluorescein isothiocyanate-labeled goat antimouse IgG.
Figure 3 Immunohistochemical detection of FAP using mAb F19 in a colon carcinoma (A) or matched control normal colon tissue from the same patient (B).
Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human FAP
- Species Reactivity
- Human
- Clone
- F19
- Applications
- ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
- Related Disease
- Tumor
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the Certificate of Analysis for the lot-specific concentration.
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody can be used in Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoassay, Radioimmunoassay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, and Blocking. Optimal working dilutions should be determined experimentally by the investigator.
Target
- Alternative Names
- FAPA; SIMP; DPPIV; FAPalpha; prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; dipeptidyl peptidase FAP; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; seprase; surface-expressed protease
- Gene ID
- 2191
- UniProt ID
- Q12884
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Fap"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-211 | Anti-Human FAP Recombinant Antibody (TAB-211) | ELISA, IP, FC, FuncS, Neut, IF, WB | IgG1 - kappa |
Customer Reviews and Q&As
Achieved High Sensitivity
Worked with Automation
Stable and Reliable
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-A1368-YJ, RRID: AB_3111429)
Popular products with customers
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, ELISA, FuncS
Application: Depletion, FuncS
Application: SPR, Inhib, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
For Research Use Only. Not For Clinical Use.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.