Rabbit Anti-FAS Recombinant Antibody (clone 2B6) (CAT#: ZG-0664J)
This product is a rabbit antibody that recognizes FAS.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-FAS Antibody (ZG-0664J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate, U87 whole cell lysate
All lanes: FAS antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 12, 10, 17, 15, 36, 25 kDa
Observed band size: 45 kDa
IHC
Figure 2 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Fas
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FAS
- Species Reactivity
- Human
- Clone
- 2B6
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Tumor
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
Related Resources
TNF Signaling Pathway
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-094 | Human Anti-FAS Recombinant Antibody (clone HFE7A); scFv Fragment | ELISA, WB, IF, FuncS | Human scFv |
| PSBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); scFv Fragment | WB, IF, FuncS | Human scFv |
| PSBL-724 | Human Anti-FAS Recombinant Antibody (clone EP6b-B01); scFv Fragment | WB, FuncS | Human scFv |
| HPAB-0035-YJ-S(P) | Human Anti-FAS Recombinant Antibody (clone h-HFE7A); scFv Fragment | ELISA, Cyt, Apop | Human scFv |
| HPAB-AP649-YC-S(P) | Human Anti-FAS Recombinant Antibody (clone F45D9-γ4); scFv Fragment | IHC, FC, ADCC, CDC, FuncS | Human scFv |
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