Rabbit Anti-FAS Recombinant Antibody (clone 2B6) (CAT#: ZG-0664J)

Figure 1 Rabbit Anti-FAS Antibody (ZG-0664J) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate, U87 whole cell lysate
All lanes: FAS antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 12, 10, 17, 15, 36, 25 kDa
Observed band size: 45 kDa

Figure 2 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.