Rabbit Anti-FAS Recombinant Antibody (clone 2B6) (CAT#: ZG-0664J)

This product is a rabbit antibody that recognizes FAS.

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  • Tested Data
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  • COA
WB

Figure 1 Rabbit Anti-FAS Antibody (ZG-0664J) in WB

Figure 1 Rabbit Anti-FAS Antibody (ZG-0664J) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate, U87 whole cell lysate
All lanes: FAS antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 12, 10, 17, 15, 36, 25 kDa
Observed band size: 45 kDa

IHC

Figure 2 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC

Figure 2 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC

Figure 3 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC

Figure 3 Rabbit Anti-FAS Antibody (ZG-0664J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human Fas
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human FAS
  • Species Reactivity
  • Human
  • Clone
  • 2B6
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Uconjugated
  • Related Disease
  • Tumor

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
    WB:1:500-1:5000, IHC:1:50-1:200

Target

  • Alternative Names
  • APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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