Rabbit Anti-FGFR3 Recombinant Antibody (clone 5B6)
CAT#: ZG-0667J
This product is a rabbit antibody that recognizes FGFR3.
Tested Data
Gene Expression
IF
Figure 1 Rabbit Anti-FGFR3 Antibody (ZG-0667J) in IF
Immunofluorescence staining of HepG2 Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67204/1419_D10_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67204/1382_D10_2_blue_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4231/11838_B_7_5.jpg
Normal Tissue
Figure 4 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4231/11838_A_6_6.jpg
Normal Tissue
Figure 5 Skin
Cells in basal layer
Staining: High
Intensity: Strong
Quantity:>75%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4231/11838_B_8_1.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CACO-2, HaCaT, Hep G2, NTERA-2, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000068078-FGFR3
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Specifications
- Immunogen
- A synthesized peptide derived from human FGFR3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FGFR3
- Species Reactivity
- Human
- Clone
- 5B6
- Applications
- ELISA, IF
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunofluorescence.
IF:1:20-1:200
Target
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Related Diseases
Bladder Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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