Rabbit Anti-GSK3B Recombinant Antibody (clone 3A6) (CAT#: ZG-0697J)
This product is a rabbit antibody that recognizes GSK3B.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-GSK3B Antibody (ZG-0697J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-GSK3B antibody at 0.77μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 KDa
Observed band size: 46 KDa
IHC
Figure 2 Rabbit Anti-GSK3B Antibody (ZG-0697J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-GSK3B Antibody (ZG-0697J) in IF
Immunofluorescence staining of Hela cells (treated with 50mM Calyculin A for 30min) with this product at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-GSK3B (Ser9)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GSK3B
- Species Reactivity
- Human
- Clone
- 3A6
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Uconjugated
- Related Disease
- Parkinson disease and Alzheimer disease
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- glycogen synthase kinase-3 beta; GSK-3 beta; serine/threonine-protein kinase GSK3B; GSK3 β
- Gene ID
- 2932
- UniProt ID
- P49841
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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