Does this antibody block IL6 activity?

Yes, this Human Anti-IL6 Recombinant Antibody is validated for Blocking assays. It binds to human IL6 and can inhibit its interaction with the IL6 receptor.

What species does this antibody react with?

In addition to Human IL6, this antibody also reacts with Cynomolgus monkey, Rat, and Mouse IL6. This cross-reactivity makes it a versatile tool for inflammation research.

Human Anti-IL6 Recombinant Antibody (HPAB-S0077-YC)

CAT#: HPAB-S0077-YC

This product is a recombinant human antibody that recognizes IL6. The human IL-6 protein was used as an immunogen. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Human Anti-IL6 Recombinant Antibody (HPAB-S0077-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-S0077-YC in non-reduced and β-mercaptoethanol-reduced conditions.

SEC-HPLC

Figure 2 Human Anti-IL6 Recombinant Antibody (HPAB-S0077-YC) in HPLC

The purity of HPAB-S0077-YC was greater than 95% as determined by SEC-HPLC.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg

Normal Tissue Figure 4 Urinary bladder

Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg

Normal Tissue Figure 5 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6

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Specifications

Immunogen
Human IL-6

Host Species
Human

Derivation
Humanized

Type
Humanized IgG

Specificity
Human IL6

Species Reactivity
Human, Cynomolgus, Rat, Mouse

Applications
ELISA, Block

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
IL6; interleukin 6; HGF; HSF; BSF2; IL-6; IFNB2; interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor

Gene ID
3569

UniProt ID
P05231

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Breast cancer biomarkers at key points during disease progression

Effective IL6 Blocker

We used this human recombinant antibody to block IL6 signaling in our cell culture models. It was very effective in preventing the downstream inflammatory response. The antibody is pure and preservative-free, which is ideal for functional assays. It reacts well with human IL6.

High Specificity ELISA

This antibody serves as an excellent capture reagent in our IL6 ELISA. It binds human IL6 with high affinity and specificity. We also appreciate that it works with cynomolgus and mouse samples, adding flexibility to our research. The batch consistency has been excellent.

Q&As

Does this antibody block IL6 activity?

A: Yes, this Human Anti-IL6 Recombinant Antibody is validated for Blocking assays. It binds to human IL6 and can inhibit its interaction with the IL6 receptor.
What species does this antibody react with?

A: In addition to Human IL6, this antibody also reacts with Cynomolgus monkey, Rat, and Mouse IL6. This cross-reactivity makes it a versatile tool for inflammation research.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-S0077-YC, RRID: AB_3713897)

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Related Diseases

Inflammatory Bowel Diseases

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Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-S0077-YC.pdf

MSDS

MSDS - HPAB-S0077-YC.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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