Mouse Anti-MUC1 Recombinant Antibody (clone AR20.5) (CAT#: PABC-399)

This product is a recombinant mouse antibody that can recognize MUC1. AR20.5 bound to the MUC1 peptide with a KD of 880 ± 123 nm. AR20.5 binds a 6-amino acid epitope (DTRPAP) within the repeating segment of 20 amino acids in the MUC1 VNTR domain. The AR20.5 epitope contains a single putative glycosylation site at Thr.

Specific Inquiry Online Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
ELISPOT

Figure 1 Interferon (IFN)-γ enzyme-linked immunospot (ELISPOT) data from patients treated with BrevaRex mAb-AR20.5.

Figure 1 Interferon (IFN)-γ enzyme-linked immunospot (ELISPOT) data from patients treated with BrevaRex mAb-AR20.5.

T-cell responses to MUC1 pre- and post-mAb-AR20.5 treatment were assessed for production of IEN-γ by ELISPOT assay in response to stimulation with 31mer MUCI peptide. PBMC obtained from heparinized blood of patients before and after administration of mAb-AR20.5 were isolated on Ficoll-Hypaque gradients and cryopreserved. Cells from pre- and post therapy samples were thawed, washed and stimulated with the 31mer MUCI peptide for 1 week, using autologous PBMC as antigen presenting cells. Cultures were then restimulated for 24 h with MUC1 peptide-pulsed autologous dendritic cells generated in parallel with the stimulated PBMC cultures. MUC1-specific T cells were quantified in an IFN-γ ELISPOT.

De Bono, J. S., Rha, S., Stephenson, J., Schultes, B. C., Monroe, P., Eckhardt, G. S., ... & Rowinsky, E. K. (2004). Phase I trial of a murine antibody to MUC1 in patients with metastatic cancer: evidence for the activation of humoral and cellular antitumor immunity. Annals of oncology, 15(12), 1825-1833.

FuncS

Figure 2 Antitumor effect of MAb AR20.5 in CB6F1 mice.

Figure 2 Antitumor effect of MAb AR20.5 in CB6F1 mice.

CB6Fl mice were immunized four times with MAb AR20.5 or controls (three times prior to tumor inoculation (Days 0.7. and 14), once post-tumor inoculation (Day 28) with 50 ug/mouse, i.v.). Tumors were induced by iniection of 413BCR cells into the tail vein (Day 21). The mice were observe for distress and weight loss and sacrificed when they became moribund. Survival times are plotted for each group (n= 5) and p values calculated by log-rank test.

Qi, W., Schultes, B. C., Liu, D., Kuzma, M., Decker, W., & Madiyalakan, R. (2001). Characterization of an anti-MUC1 monoclonal antibody with potential as a cancer vaccine. Hybridoma and hybridomics, 20(5-6), 313-324.

FuncS

Figure 3 CDC and ADCC properties of MAb AR20.5.

Figure 3 CDC and ADCC properties of MAb AR20.5.

ZR75-1 cells were labeled with ⁵¹Cr and incubated with MAb AR20.5 or MAb AR20.5 (IgM) or control IgG, (mouse IgG1, MOPC-21) in a range of 0.625 to 20 ug/mL for 1 h at room temperature. Cells were washed once and then incubated with either 15% fresh human serum in complete RPMI 1640 media for 4 h for CDC (A) or with human PBMC in an effector: target ratio of 50:1 for ADCC (B). Supernatants were harvested and tested for ⁵¹Cr release. Specific lysis was calculated by subtracting the spontaneous release from each sample and dividing the results by the corrected makimum release by ZR75-1 cells in the presence of Triton X-100. Mouse IgG1 was used as negative control.

Qi, W., Schultes, B. C., Liu, D., Kuzma, M., Decker, W., & Madiyalakan, R. (2001). Characterization of an anti-MUC1 monoclonal antibody with potential as a cancer vaccine. Hybridoma and hybridomics, 20(5-6), 313-324.


Specifications

  • Immunogen
  • MUC1 from an ovarian cancer patient
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human MUC1
  • Species Reactivity
  • Human
  • Clone
  • AR20.5
  • Applications
  • ELISA, FC, FuncS

Product Property

  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.

Target

  • Alternative Names
  • EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone AR20.5"

See other products for "MUC1"

Immunotoxin

CAT Product Name Application Type
AGTO-L036E anti-MUC1 immunotoxin C242 (Fab)-PE Cytotoxicity assay, Functional assay
AGTO-L064E anti-MUC1 immunotoxin H23 (scFv)-PE Cytotoxicity assay, Functional assay

Human Antibody

CAT Product Name Application Type
TAB-0278CL Human Anti-MUC1 Recombinant Antibody (TAB-0278CL) ELISA, Internalization, BI, FuncS Human IgG
TAB-431MZ Anti-Human MUC1 Recombinant Antibody (PH1) ELISA, WB, FC, IHC, SPR Human antibody
TAB-432MZ Human Anti-MUC1 Recombinant Antibody (TAB-432MZ) ELISA, FC, FACS, DB, SPR Human IgG
TAB-433MZ Human Anti-MUC1 Recombinant Antibody (TAB-433MZ) ELISA, FC, FACS, DB, SPR Human IgG
TAB-434MZ Human Anti-MUC1 Recombinant Antibody (TAB-434MZ) ELISA, FC, FACS, DB, SPR Human IgG

Fab Glycosylation

CAT Product Name Application Type
Gly-012LC Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) ELISA, FC, IHC Humanized antibody
Gly-120LC Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) ELISA Mouse antibody

Sialylated IgG Glycan

CAT Product Name Application Type
Gly-012LC-1 Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) ELISA, FC, IHC Humanized antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-109LC Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) ELISA Human antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0379MZ Chicken Anti-MUC1 Polyclonal IgY IHC, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2320 Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) IHC-P, IHC-Fr IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H56 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Pemtumomab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-H65 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Sontuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody
AFC-TAB-026ML Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Gatipotuzumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-166 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Cantuzumab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody
AFC-TAB-H77 Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) ELISA, FC, IP, FuncS, IF IgG1, κ

Submit a review or a question
There are currently no Customer reviews or questions for PABC-399. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

  • 0
  • 0
Cart
    Go to compare

    Go to compare