Mouse Anti-MUC1 Recombinant Antibody (clone B9)

Name: Mouse Anti-MUC1 Recombinant Antibody (clone B9)
SKU: HPAB-1165WJ
Rating: 5 (1 reviews)

CAT#: HPAB-1165WJ

This product is a mouse antibody targeting human MUC-1. The clone B9 reacts specifically with the MUC-1 membrane antigen expressed on MCF-7 breast adenocarcinoma cells.B9 can be identified by screening for antibodies that bind to the same epitope and by modification of the anti-MUC-1 antibody to produce libraries of modified antibody and then rescreening antibodies in the library for improved MUC-1 avidity.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg

Normal Tissue Figure 5 Stomach

Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg

Normal Tissue Figure 6 Colon

Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg

Normal Tissue Figure 7 Kidney

Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg

Normal Tissue Figure 8 Testis

Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg

RNA Expression Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1

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Specifications

Immunogen
KLH-MUC-1 synthetic peptide

Host Species
Mouse

Type
Mouse IgG

Specificity
Human MUC-1

Species Reactivity
Human

Clone
B9

Applications
ELISA, ICC

Related Disease
Cancers

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Gene ID
4582

UniProt ID
P15941

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1165WJ.pdf

MSDS

MSDS - HPAB-1165WJ.pdf

COA

Certificate of Analysis Lookup

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