Rabbit Anti-PLCG1 Recombinant Antibody (clone R81-6X-8) (CAT#: VS3-FY2113)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat PLCG1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R81-6X-8

• Applications
• Western Blot, Immunofluorescence, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• The calculated weight of the target protein is approximately 149 kDa, while the observed molecular weight stands at 149kDa.

• Related Disease
• Immune Dysregulation, Autoimmunity, And Autoinflammation and Paroxysmal Nocturnal Hemoglobinuria

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4), 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/50

• Alternative Names
• PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

• Gene ID
• 5335

• UniProt ID
• P19174

• Cellular Localization
• Cell projection

• Post Translation Modifications
• Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA ()., Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells., Ubiquitinated by CBLB in activated T-cells.

• Protein Refseq
• NP_002651.2; NP_877963.1

• Function
• Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity).
  Plays a role in actin reorganization and cell migration.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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