Anti-mucin repeats (clone ch3A5)-vc-MMAF ADC

Name: Anti-mucin repeats (clone ch3A5)-vc-MMAF ADC
SKU: ADC-220LZY
Rating: 5 (1 reviews)

CAT#: ADC-220LZY

This ADC product is composed of an anti-mucin repeats antibody (clone ch3A5) conjugated via vc linker to MMAF (ch3A5-vc-MMAF). It has demonstrated a response in ovarian cance treatment by a MOA (Mechanism of Action) of microtubules depolymerizing.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg

Normal Tissue Figure 5 Stomach

Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg

Normal Tissue Figure 6 Colon

Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg

Normal Tissue Figure 7 Kidney

Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg

Normal Tissue Figure 8 Testis

Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg

RNA Expression Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1

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Specifications

Antibody Overview
ch3A5(Chimeric Anti-mucin repeats IgG1)

Clone
ch3A5

Antibody Isotype
IgG1

Linker
VC (valine-citrulline)

Linker Class/Description
Class: Enzymatically cleavable linker-Peptide linker
Description: peptide linkers, belonging to Enzymatically cleavable linkers, combine greater systemic stability with rapid enzymatic release of the drug in the target cell. The scission of peptidic bonds relies on lysosomal proteolytic enzymes, which have very low activities in blood due to endogenous inhibitors and the unfavorably high pH value of blood.

Drug
MMAF (monomethyl auristatin F)

Drug Class/Description
Class: Auristatin
Description: Auristatins are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

Target

Introduction
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Alternative Names
MUC1; mucin 1, cell surface associated; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant;

Gene ID
4582

UniProt ID
P15941

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Datasheet

Datasheet - ADC-220LZY.pdf

MSDS

MSDS - ADC-220LZY.pdf

COA

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