What applications are suitable for the Mouse Anti-IL6 Recombinant Antibody?

This antibody is ideal for applications such as Western blotting, ELISA, and FC. It is engineered to ensure high specificity and sensitivity, making it a reliable choice for detecting IL6 in various experimental settings.

Which research areas can benefit from using this antibody?

Studies related to inflammation, immune responses, and cytokine signaling can significantly benefit. It enables researchers to explore IL6's role in physiological and pathological processes more deeply.

Can this antibody be used in flow cytometry?

While primarily designed for Western blot and ELISA, it can be adapted for flow cytometry with optimized protocols, enhancing versatility in research.

What diseases are associated with IL6 that can be explored using this antibody?

IL6 is a key player in chronic inflammatory diseases and autoimmune disorders. Research on conditions like rheumatoid arthritis, lupus, and inflammatory bowel disease can benefit from its use.

How does the antibody enhance the study of cytokine signaling pathways?

By providing specific and sensitive detection of IL6, it facilitates detailed exploration of cytokine signaling pathways, which are crucial for understanding immune responses.

Mouse Anti-IL6 Recombinant Antibody, HRP (clone 3-A4)

CAT#: VS3-XY876

Clone 3-A4 is a mouse antibody, which reacts with IL6 protein. This antibody is suitable for IL6 detection by WB, ICC, FC, ELISA assays.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg

Normal Tissue Figure 4 Urinary bladder

Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg

Normal Tissue Figure 5 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6

❮

❯

Specifications

Immunogen
recombinant protein

Host Species
Mouse

Type
Mouse IgG2b

Specificity
IL6

Species Reactivity
Human

Clone
3-A4

Applications
Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunocytochemistry

Conjugate
HRP

Product Property

Purification
Protein A affinity purified

Format
Liquid

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Store at 4°C away from light. Do not freeze.

Target

Alternative Names
CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2

Gene ID
3569

UniProt ID
P08505

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Breast cancer biomarkers at key points during disease progression

Highly Effective for ELISA

The Mouse Anti-IL6 Recombinant Antibody has been a game-changer in our lab. Its performance in ELISA was outstandingly consistent, allowing us to obtain reliable quantifications of IL6 across multiple samples. The ease with which it integrates into our existing protocols also saves a lot of time.

Excellent for Inflammatory Studies

Our study on inflammatory pathways greatly benefitted from this product; its specificity for IL6 provided clear results that enabled us to understand deeper aspects of IL6's role in inflammation without cross-reactivity issues.

Great Versatility

This antibody's versatility impressed us. We used it across different platforms including Western blot and ELISA, and it did not disappoint. Adapting it for diverse assays was a smooth process, which speaks to its thoughtfully engineered design.

Cost-Effective for Labs

Considering the reliability and performance, this antibody is a cost-effective solution for labs needing consistent results over long-term projects.

Facilitates Understanding of Cytokine Pathways

This product has enhanced our ability to delve into cytokine signaling pathways, providing clear insights into IL6 dynamics that were previously hard to achieve.

Q&As

What applications are suitable for the Mouse Anti-IL6 Recombinant Antibody?

A: This antibody is ideal for applications such as Western blotting, ELISA, and FC. It is engineered to ensure high specificity and sensitivity, making it a reliable choice for detecting IL6 in various experimental settings.
Which research areas can benefit from using this antibody?

A: Studies related to inflammation, immune responses, and cytokine signaling can significantly benefit. It enables researchers to explore IL6's role in physiological and pathological processes more deeply.
Can this antibody be used in flow cytometry?

A: While primarily designed for Western blot and ELISA, it can be adapted for flow cytometry with optimized protocols, enhancing versatility in research.
What diseases are associated with IL6 that can be explored using this antibody?

A: IL6 is a key player in chronic inflammatory diseases and autoimmune disorders. Research on conditions like rheumatoid arthritis, lupus, and inflammatory bowel disease can benefit from its use.
How does the antibody enhance the study of cytokine signaling pathways?

A: By providing specific and sensitive detection of IL6, it facilitates detailed exploration of cytokine signaling pathways, which are crucial for understanding immune responses.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Inflammatory Bowel Diseases

Rheumatoid Arthritis

Graft-versus-Host Disease

AGE-RAGE Signaling Pathway in Diabetic Complications

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-XY876.pdf

MSDS

MSDS - VS3-XY876.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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HPAB-0017-YC-S(P)	Human Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-0017-YC-S(P))	ELISA, Block	Human scFv
HPAB-0876-FY-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-0876-FY-F(E))	ELISA	Human Fab
HPAB-1562-FY-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-1562-FY-F(E))	WB, ELISA, Neut	Humanized Fab
HPAB-1563-FY-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-1563-FY-F(E))	WB, ELISA, Neut	Humanized Fab
HPAB-1564-FY-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-1564-FY-F(E))	WB, ELISA, Neut	Humanized Fab

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NS-043CN	Human Anti-IL6 Recombinant Antibody (NS-043CN)	ELISA, WB	Human IgG1
NS-043CN-F(E)	Human Anti-IL6 Recombinant Antibody; Fab Fragment (NS-043CN-F(E))	ELISA, WB	Human Fab
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HPAB-S0169-YC	Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0169-YC)	ELISA, Inhib	Camelid VHH
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VS-0425-FY11	Human Anti-IL6 (clone BA001) scFv-Fc Chimera	ELISA, Block	Human IgG1, scFv-Fc

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VS-0425-YC526	Recombinant Anti-IL6 Vesicular Antibody, EV Displayed (VS-0425-YC526)	ELISA, FC, Neut, Cell-uptake

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VS-0525-XY3537	Anti-Mouse IL6 Immunohistochemistry Kit	IHC
VS-0525-XY3538	Anti-Human IL6 Immunohistochemistry Kit	IHC

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