Rabbit Anti-Raf1 (phospho S621) Monoclonal Antibody
CAT#: VS-0126-XL102
Specifically identifying Raf1 phosphorylated at Serine 621, this rabbit monoclonal offers precise recognition of this essential catalytic stability site. It is an ideal laboratory tool for kinase activity studies in WB and IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2639/1867_B3_30_cr5b68189b791a9_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_5_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19291/43320_A_5_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132155-RAF1
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Specifications
- Host Species
- Rabbit
- Specificity
- Raf1 (phospho S621)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Derived by utilizing Protein A-linked stationary phases to secure a highly refined and professionally purified monoclonal antibody preparation.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
IHC: 1:50-1:200
Target
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
TCR Signaling Pathway
Related Diseases
Bladder Cancer
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Hepatocellular Carcinoma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "RAF1"
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| CAT | Product Name | Application | Type |
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| MOB-1388CT | Recombinant Mouse anti-Human RAF1 Monoclonal antibody (520) | WB |
| CAT | Product Name | Application | Type |
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| BRD-0796MZ | Chicken Anti-c-Raf Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| MOR-2982 | Hi-Affi™ Recombinant Rabbit Anti-RAF1 Monoclonal Antibody (DS2982AB) | IP, IHC-P, ICC, IF, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1316-CN | Recombinant Rabbit Anti-RAF1 Monoclonal Antibody (CBACN-476) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2367-CN | Recombinant Rabbit Anti-RAF1 (phosphorylated Ser259) Monoclonal Antibody (CBACN-653) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2368-CN | Recombinant Rabbit Anti-RAF1 (phosphorylated Ser43) Monoclonal Antibody (CBACN-654) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2369-CN | Recombinant Rabbit Anti-RAF1 (phosphorylated Ser621) Monoclonal Antibody (CBACN-655) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY138 | Mouse Anti-NHP RAF1 Recombinant Antibody (clone 4G4) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1866 | Anti-RAF1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5965 | Anti-Mouse RAF1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5964 | Anti-Human RAF1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5966 | Anti-Rat RAF1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL97 | Rabbit Anti-Raf1 (phospho S259) Monoclonal Antibody (RCB-P0935) | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL209 | Rabbit Anti-Raf1 (phospho S259) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL213 | Rabbit Anti-Raf1 (phospho S43) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
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