Recombinant Mouse anti-Human RAF1 Monoclonal antibody (520)
CAT#: MOB-1388CT
Recombinant Mouse monoclonal antibody to Human RAF1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2639/1867_B3_30_cr5b68189b791a9_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_5_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19291/43320_A_5_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132155-RAF1
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Specifications
- Immunogen
- Purified recombinant human Raf-1 (mw:75 kDa).
- Host Species
- Mouse
- Specificity
- This antibody is specifically reactive to Raf-1 N-terminal (1-240 a.a.)
- Species Reactivity
- Human
- Clone
- 520
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- n/a
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; Oncogene RAF1; proto-oncogene c-RAF; C-Raf proto-oncogene, serine/threonine kinase; v-raf-1 murine leukemia viral oncogene homolog 1; raf proto-oncogene serine/threonine protein kinase;
- Gene ID
- 5894
- UniProt ID
- P04049
- Protein Refseq
- NP_002871
- Function
- ATP binding; MAP kinase kinase kinase activity; Ras GTPase binding; metal ion binding; mitogen-activated protein kinase kinase binding; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase activity; protein serine/threo
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
TCR Signaling Pathway
Related Diseases
Bladder Cancer
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Hepatocellular Carcinoma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "RAF1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0796MZ | Chicken Anti-c-Raf Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS3-QX955 | Mouse Anti-RAF1 Recombinant Antibody (clone 4G4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ323 | Rabbit Anti-RAF1 Recombinant Antibody (VS3-CJ323) | WB | Rabbit IgG |
| VS3-FY1244 | Recombinant Rabbit Anti-RAF1 Antibody (clone R05-9B1) | WB, ICC, IF | Rabbit IgG |
| VS3-FY1245 | Recombinant Rabbit Anti-RAF1 (phospho Ser259) Antibody (clone R07-4J4) | WB, IHC-P | Rabbit IgG |
| VS3-WK2158 | Rabbit Anti-RAF1 (pS621) Recombinant Antibody (clone R09-8G1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY138 | Mouse Anti-NHP RAF1 Recombinant Antibody (clone 4G4) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1866 | Anti-RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5965 | Anti-Mouse RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5964 | Anti-Human RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5966 | Anti-Rat RAF1 Immunohistochemistry Kit | IHC |
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