Recombinant Mouse anti-Human RAF1 Monoclonal antibody (520) (CAT#: MOB-1388CT)
Recombinant Mouse monoclonal antibody to Human RAF1, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2639/1867_B3_30_cr5b68189b791a9_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2639/1867_B3_30_cr5b68189b791a9_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_5_3_selected.jpg
(Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_5_3_selected.jpg
Figure 3 IHC staining of human gall bladder
(Immunohistochemical staining of human gall bladder shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19291/43320_A_5_4_selected.jpg
(Immunohistochemical staining of human gall bladder shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19291/43320_A_5_4_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_8.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_7_3.jpg
Figure 6 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_5.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132155-RAF1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132155-RAF1
Specifications
- Immunogen
- Purified recombinant human Raf-1 (mw:75 kDa).
- Host Species
- Mouse
- Specificity
- This antibody is specifically reactive to Raf-1 N-terminal (1-240 a.a.)
- Species Reactivity
- Human
- Clone
- 520
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- n/a
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; Oncogene RAF1; proto-oncogene c-RAF; C-Raf proto-oncogene, serine/threonine kinase; v-raf-1 murine leukemia viral oncogene homolog 1; raf proto-oncogene serine/threonine protein kinase;
- Gene ID
- 5894
- UniProt ID
- P04049
- Protein Refseq
- NP_002871
- Function
- ATP binding; MAP kinase kinase kinase activity; Ras GTPase binding; metal ion binding; mitogen-activated protein kinase kinase binding; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase activity; protein serine/threo
Related Resources
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
TCR Signaling Pathway
Bladder Cancer
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Hepatocellular Carcinoma
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
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| BRD-0796MZ | Chicken Anti-c-Raf Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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