Mouse Anti-NHP RAF1 Recombinant Antibody (clone 4G4)
CAT#: VS-1024-XY138
Featuring a mouse-derived monoclonal formulation, this antibody is tailored for the detection of RAF1, with reactivity in human, non-human primate, and rat specimens. The use of a purified recombinant fragment of human RAF1 as the immunogen guarantees precise targeting. It is suited for multiple applications, including Western blot (WB), immunohistochemistry (IHC), flow cytometry (FC), and ELISA, positioning it as a vital tool for detailed RAF1 research and analysis across various platforms.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2639/1867_B3_30_cr5b68189b791a9_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_5_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19291/43320_A_5_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132155-RAF1
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Specifications
- Immunogen
- Purified recombinant fragment of human RAF1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- RAF1
- Species Reactivity
- Human, Non-human primate, Rat
- Clone
- 4G4
- Applications
- WB, IHC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
TCR Signaling Pathway
Related Diseases
Bladder Cancer
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Hepatocellular Carcinoma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 4G4"
- CAT
- Product Name
See other products for "RAF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0796MZ | Chicken Anti-c-Raf Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2982 | Hi-Affi™ Recombinant Rabbit Anti-RAF1 Monoclonal Antibody (DS2982AB) | IP, IHC-P, ICC, IF, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1316-CN | Recombinant Rabbit Anti-RAF1 Monoclonal Antibody (CBACN-476) | WB, IF | Rabbit IgG |
| MRO-2367-CN | Recombinant Rabbit Anti-RAF1 (phosphorylated Ser259) Monoclonal Antibody (CBACN-653) | WB, IHC | Rabbit IgG |
| MRO-2368-CN | Recombinant Rabbit Anti-RAF1 (phosphorylated Ser43) Monoclonal Antibody (CBACN-654) | WB | Rabbit IgG |
| MRO-2369-CN | Recombinant Rabbit Anti-RAF1 (phosphorylated Ser621) Monoclonal Antibody (CBACN-655) | WB | Rabbit IgG |
| ZG-0539U | Rabbit Anti-Phospho-RAF1 (S621) Recombinant Antibody (clone 1C2) | ELISA, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1866 | Anti-RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5965 | Anti-Mouse RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5964 | Anti-Human RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5966 | Anti-Rat RAF1 Immunohistochemistry Kit | IHC |
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