Recombinant Rabbit Anti-BSG Antibody (clone R04-5B3)
CAT#: VS3-FY169
This product is a recombinant rabbit antibody that recognizes human BSG protein. It can detect a band of approximately 42 kDa (observedd molecular weight: 50 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74870/1466_A2_3_selected.jpg
Normal Tissue
Figure 2 Testis
Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_4_6.jpg
Normal Tissue
Figure 3 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_B_6_6.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172270-BSG
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ICC
Figure 1. Recombinant Rabbit Anti-BSG Antibody (clone R04-5B3) in ICC.
Immunocytochemical analysis of CD147 (green) in HeLa using CD147 antibody and DAPI (blue).
ICC
Figure 2. Recombinant Rabbit Anti-BSG Antibody (clone R04-5B3) in ICC.
Immunocytochemical analysis of CD147 (green) in HeLa using CD147 antibody and DAPI (blue).
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Specifications
- Immunogen
- A synthetic peptide of human CD147
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BSG
- Species Reactivity
- Human
- Clone
- R04-5B3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 42 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BSG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B050(A) | Recombinant Anti-human BSG Intrabody [(D-Arg)9] | WB, ICC, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B050(G) | Recombinant Anti-human BSG Intrabody [+36 GFP] | IF, FC, FuncS | scFv-(+36GFP) |
| IAB-B050(T) | Recombinant Anti-human BSG Intrabody [Tat] | WB, ICC, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-156 | Anti-Human CD147 Recombinant Antibody (TAB-156) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-082 | Human Anti-BSG Recombinant Antibody (clone 4A5) | WB, ELISA, FuncS | Human IgG |
| HPAB-0126CQ | Mouse Anti-BSG Recombinant Antibody (clone HAb18) | ELISA, IF, WB | Mouse IgG1 |
| VS3-FY241 | Recombinant Mouse Anti-BSG Antibody (clone 2D3A9) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
| VS3-FY242 | Recombinant Mouse Anti-BSG Antibody (clone 2D8C11) | ELISA, IHC, FC | Mouse IgG2b |
| VS3-FY243 | Recombinant Mouse Anti-BSG Antibody (clone 5A6F8) | ELISA, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-461 | Human Anti-BSG Recombinant Antibody (clone 5F6); Fab Fragment | WB, FuncS | Chimeric (mouse/human) Fab |
| FAMAB-0033CQ-F(E) | Mouse Anti-BSG Recombinant Antibody Fab Fragment (FAMAB-0033CQ-F(E)) | ELISA, Block | Mouse Fab |
| FAMAB-0034CQ-F(E) | Human Anti-BSG Recombinant Antibody Fab Fragment (FAMAB-0034CQ-F(E)) | ELISA, Block | Chimeric (mouse/human) Fab |
| HPAB-3079LY-F(E) | Mouse Anti-BSG Recombinant Antibody; Fab Fragment (HPAB-3079LY-F(E)) | IHC, Inhib | Mouse Fab |
| HPAB-3080LY-F(E) | Human Anti-BSG Recombinant Antibody; Fab Fragment (HPAB-3080LY-F(E)) | IHC, Inhib | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-461 | Mouse Anti-BSG Recombinant Antibody (clone 5F6); scFv Fragment | WB, FuncS | Mouse scFv |
| PSBC-403 | Mouse Anti-BSG Recombinant Antibody (clone 6H8); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-M0438-YC-S(P) | Human Anti-BSG Recombinant Antibody; scFv Fragment (HPAB-M0438-YC-S(P)) | ELISA, IHC, Inhib | Human scFv |
| HPAB-M0439-YC-S(P) | Human Anti-BSG Recombinant Antibody; scFv Fragment (HPAB-M0439-YC-S(P)) | ELISA | Human scFv |
| HPAB-M0440-YC-S(P) | Human Anti-BSG Recombinant Antibody; scFv Fragment (HPAB-M0440-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1285CL | Anti-Human BSG Recombinant Antibody (2.6.1 (ABX-RB2)) | Inhib | Fully human antibody |
| TAB-1287CL | Human Anti-BSG Recombinant Antibody (TAB-1287CL) | Cyt, FC | Human IgG |
| TAB-1288CL | Human Anti-BSG Recombinant Antibody (TAB-1288CL) | Cyt, FC | Human IgG |
| TAB-1289CL | Human Anti-BSG Recombinant Antibody (TAB-1289CL) | Cyt, FC | Human IgG |
| TAB-1290CL | Human Anti-BSG Recombinant Antibody (TAB-1290CL) | Cyt, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-070CQ | Human Anti-BSG Recombinant Antibody (clone Ab-1) | ELISA, Block | Human IgG1, κ |
| NEUT-071CQ | Mouse Anti-BSG Recombinant Antibody (clone HIM6) | FC, WB, IHC, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0389 | Hi-Affi™ Rabbit Anti-BSG Recombinant Antibody (clone DS389AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-020CN | Mouse Anti-BSG Recombinant Antibody (clone 5F6) | ELISA, Block, FuncS | Mouse IgG1 |
| NS-020CN-F(E) | Mouse Anti-BSG Recombinant Antibody (clone 5F6); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-156 | Afuco™ Anti-BSG ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-156) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-883 | Afuco™ Anti-BSG ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-883) | IF, IP, Neut, FuncS, ELISA | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1484 | AbPlus™ Anti-BSG Magnetic Beads (VS-0724-YC1484) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY33 | Human Anti-BSG (clone HAb18) scFv-Fc Chimera | ELISA, IF, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC238 | Recombinant Anti-BSG Vesicular Antibody, EV Displayed (VS-0425-YC238) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY837 | Anti-Mouse BSG Immunohistochemistry Kit | IHC | |
| VS-0525-XY838 | Anti-Rat BSG Immunohistochemistry Kit | IHC |
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