Recombinant Mouse Anti-IL6 Capture Antibody (clone 2B5)
CAT#: VS3-WK1642
This antibody is a mouse recombinant antibody against human IL6. The sandwich ELISA was established using matched antibody pair 2B5 and 6B8. The clone 2B5 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human IL6 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg
Normal Tissue
Figure 4 Urinary bladder
Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6
❮
❯
Specifications
- Immunogen
- Recombinant human IL6 protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human IL6
- Species Reactivity
- Human
- Clone
- 2B5
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Interleukin 6
- Gene ID
- 3569
- UniProt ID
- P05231
- Long Name
- Interleukin 6
- Sequence Similarities
- Belongs to the IL-6 superfamily.
- Cellular Localization
- Secreted
- Post Translation Modifications
- N- and O-glycosylated.
- Protein Refseq
- NP_000591.1
- Function
- Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).
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Related Diseases
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-253 | Anti-Human IL-6 Recombinant Antibody (Olokizumab) | WB, ELISA, FC, IP, FuncS, IF, Neut | IgG4 - kappa |
| TAB-0172CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0172CL-S(P)) | ELISA, Block, In vivo | Human scFv |
| TAB-0173CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0173CL-S(P)) | ELISA, Block, In vivo | Human scFv |
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| CAT | Product Name | Application | Type |
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| MOB-0249MC | Rabbit Anti-IL6 Antibody | WB, ELISA | |
| HPAB-0089-WJ | Human Anti-IL6 Recombinant Antibody (HPAB-0089-WJ) | ELISA, WB | Humanized IgG |
| HPAB-1250-FY | Mouse Anti-IL6 Recombinant Antibody (HPAB-1250-FY) | ELISA | Mouse IgG |
| HPAB-1251-FY | Mouse Anti-IL6 Recombinant Antibody (HPAB-1251-FY) | ELISA | Mouse IgG |
| HPAB-1252-FY | Mouse Anti-IL6 Recombinant Antibody (HPAB-1252-FY) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-613 | Recombinant Human Anti-IL-6 Antibody Fab Fragment | ELISA, Neut | Fab |
| HPAB-0017-YC-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-0017-YC-F(E)) | ELISA, Block | Human Fab |
| HPAB-0824-CN-F(E) | Human Anti-IL6 Recombinant Antibody (clone Ab18); Fab Fragment | ELISA | Human Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0026CL | Human Anti-IL6 Recombinant Antibody (TAB-0026CL) | Elisa, In vivo, FuncS, Block | Chimeric (mouse/human) IgG |
| TAB-0026CL-S(P) | Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0026CL-S(P)) | Elisa, In vivo, FuncS, Block | Mouse scFv |
| TAB-0026CL-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (TAB-0026CL-F(E)) | Elisa, In vivo, FuncS, Block | Chimeric (mouse/human) Fab |
| TAB-670LC-S(P) | Anti-human IL6 scFv Fragment (NTS) | ELISA | Chimeric antibody (mouse/human) |
| VS3-WK1519 | Human Anti-IL6 Recombinant Antibody (clone CLLB8) | ELISA, FC | Chimeric (mouse/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0174CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0174CL-S(P)) | ELISA, Block, In vivo | Human scFv |
| TAB-0175CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0175CL-S(P)) | ELISA, Block, In vivo | Human scFv |
| TAB-0176CL-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0176CL-S(P)) | ELISA, Block, In vivo | Human scFv |
| TAB-671LC-S(P) | Anti-human IL6 scFv Fragment (SK2a) | ELISA | Human antibody |
| TAB-672LC-S(P) | Anti-human IL6 scFv Fragment (SK2b) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-037LC | Recombinant Anti-Human IL6 Antibody (Fab glycosylation) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-197LC | Recombinant Anti-Human IL6 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0284MZ | Chicken Anti-Interleukin-6 Polyclonal IgY | WB, Indirect ELISA | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1472CQ | Mouse Anti-IL6 Recombinant Antibody (clone AS16) | IHC, IP, Neut | Mouse IgG1 |
| NEUT-1473CQ | Rat Anti-Il6 Recombinant Antibody (clone 20F3) | Block, ELISA, IHC-P, IP, Neut, WB | Rat IgG1 |
| NEUT-1475CQ | Rat Anti-Il6 Recombinant Antibody (NEUT-1475CQ) | Neut | Rat IgG2a |
| NEUT-1476CQ | Rat Anti-Il6 Recombinant Antibody (clone CBL112) | Neut, WB | Rat IgG1 |
| NEUT-1477CQ | Rat Anti-Il6 Recombinant Antibody (clone CBL879) | FC, IHC, IP, Neut | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1474CQ | Rat Anti-Il6 Recombinant Antibody (clone 32C11) | Block, ELISA, WB | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1814 | Rabbit Anti-IL6 Recombinant Antibody (clone DS1814AB) | WB | Rabbit IgG |
| MOR-4277 | Rabbit Anti-IL6 Recombinant Antibody (clone SI360DS) | ELISA | Rabbit IgG |
| MOR-4622 | Rabbit Anti-IL6 Recombinant Antibody (clone TH135DS) | WB, ELISA | Rabbit IgG |
| MOR-4623 | Rabbit Anti-IL6 Recombinant Antibody (clone TH136DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3429 | PE-B8/Human IL6 (ILKQKIALD) MHC Tetramer | FCM | |
| MHC-LC3430 | APC-B8/Human IL6 (ILKQKIALD) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-043CN | Human Anti-IL6 Recombinant Antibody (NS-043CN) | ELISA, WB | Human IgG1 |
| NS-043CN-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (NS-043CN-F(E)) | ELISA, WB | Human Fab |
| NS-043CN-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (NS-043CN-S(P)) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0824-CN-S(P) | Human Anti-IL6 Recombinant Antibody (clone Ab18); scFv Fragment | ELISA | Human scFv |
| HPAB-0825-CN-S(P) | Human Anti-IL6 Recombinant Antibody (clone 18E); scFv Fragment | ELISA | Human scFv |
| HPAB-0877-FY-F(E) | Human Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-0877-FY-F(E)) | ELISA | Human Fab |
| HPAB-0089-WJ-S(P) | Human Anti-IL6 Recombinant Antibody; scFv Fragment (HPAB-0089-WJ-S(P)) | ELISA, WB | Humanized scFv |
| HPAB-1758-FY-F(E) | Rat Anti-IL6 Recombinant Antibody; Fab Fragment (HPAB-1758-FY-F(E)) | ELISA | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-239 | Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-239) | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-097 | Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-097) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-H26 | Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H26) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-253 | Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-253) | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0166-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0166-YC) | ELISA | Camelid VHH |
| HPAB-S0167-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0167-YC) | ELISA | Camelid VHH |
| HPAB-S0168-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0168-YC) | ELISA, Inhib | Camelid VHH |
| HPAB-S0169-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0169-YC) | ELISA, Inhib | Camelid VHH |
| HPAB-S0170-YC | Camelid Anti-IL6 Recombinant Single Domain Antibody (HPAB-S0170-YC) | ELISA, Inhib | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY151 | AbPlus™ Anti-IL6 Magnetic Beads (AS16) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY279 | Mouse Anti-NHP IL6 Recombinant Antibody (clone 6708) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY146 | CytoStream™ Mouse Anti-IL6 Recombinant Antibody (VS-0225-XY146) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY11 | Human Anti-IL6 (clone BA001) scFv-Fc Chimera | ELISA, Block | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC526 | Recombinant Anti-IL6 Vesicular Antibody, EV Displayed (VS-0425-YC526) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3537 | Anti-Mouse IL6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3538 | Anti-Human IL6 Immunohistochemistry Kit | IHC |
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