Recombinant Mouse Anti-IL6 Capture Antibody (clone 2B5) (CAT#: VS3-WK1642)
This antibody is a mouse recombinant antibody against human IL6. The sandwich ELISA was established using matched antibody pair 2B5 and 6B8. The clone 2B5 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human IL6 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg
(Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg
(Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg
Figure 4 Urinary bladder
(Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg
(Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg
Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6
Specifications
- Immunogen
- Recombinant human IL6 protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human IL6
- Species Reactivity
- Human
- Clone
- 2B5
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Interleukin 6
- Gene ID
- 3569
- UniProt ID
- P05231
- Long Name
- Interleukin 6
- Sequence Similarities
- Belongs to the IL-6 superfamily.
- Cellular Localization
- Secreted
- Post Translation Modifications
- N- and O-glycosylated.
- Protein Refseq
- NP_000591.1
- Function
- Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).
Recommended Products
Detection antibody
Isotype Control
Secondary Antibody
Related Resources
Inflammatory Bowel Diseases
Rheumatoid Arthritis
Graft-versus-Host Disease
AGE-RAGE Signaling Pathway in Diabetic Complications
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "IL6"
Select a product category from the dropdown menu below to view related products.
Please select product type
Mouse Antibody Products
Humanized Antibody Products
IgG Antibody Products
Chimeric Antibody Products
Human Antibody Products
Fab Glycosylation Antibody Products
Deglycosylated Antibody Products
Chicken IgY Antibody Products
Neutralizing Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
MHC Tetramer Products for Cancer
Neuroscience Antibody Products
ScFv Antibody Products
ADCC Enhanced Antibody Products
Fab Antibody Products
Single Domain Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
ScFv-Fc Chimera Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-239 | Anti-Human IL6 Recombinant Antibody (TAB-239) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 |
| TAB-0171CL | Mouse Anti-IL6 Recombinant Antibody (TAB-0171CL) | ELISA, Block, In vivo | Mouse IgG |
| TAB-669LC | Anti-human IL6 Recombinant Antibody (SK2) (TAB-669LC) | Inhib | |
| TAB-673LC | Anti-human IL6 Recombinant Antibody | ELISA, FC | |
| TAB-679LC | Anti-human IL6 Recombinant Antibody (MH166) (TAB-679LC) | ELISA, Inhib, FuncS |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-WK1642. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, Inhib, ICC, WB
-5.jpg)
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, Inhib, FuncS
Application: WB, ELISA, FC, IHC, IF, IP
Application: ELISA, FC, Inhib
-2.png)
Application: ELISA
-2.png)
Application: WB, ELISA, FuncS
Application: ELISA, Inhib, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
