Anti-Human CD19 Recombinant Antibody (MDX-1342) (CAT#: TAB-108CL)
Recombinant Human monoclonal antibody expressed in CHO binding to Human CD19. MDX-1342 is a fully human monoclonal antibody (HuMAb) with enhanced antibody-dependent cellular cytotoxicity (ADCC) effector function, targeting CD19-membrane receptor which is highly expressed on malignant chronic lymphocytic leukemia (CLL) cells.
Specific Inquiry
Figure 1 Oligosaccharide analysis of MDX-1342 and parental anti-CD19 antibody by capillary electrophoresis with laser induced fluorescence detection; assignment of carbohydrate structures by comparison with glycan standards.
Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.
FC
Figure 2 FACS titration of MDX-1342 binding on human FcRγIIIa transfected CHO-S cells (a), human lymphoma cell lines (b), and FcRγIIIa-Val158-transfected and FcRIIIa-Phe158-transfected CHO cells (c)
Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.
ADCC
Figure 3 Comparison of ADCC (a) and phagocytosis (b) mediated by MDX-1342 and controls
Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.
FuncS
Figure 4 Kaplan–Meier plot of the Ramos systemic tumor model treated with MDX-1342 at 0.03, 0.3, 3, and 30 mg/kg
Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.
FC
Figure 5 FACS binding of MDX-1342 to CD40+ human B cells (a) and cynomolgus monkey CD40+ B cells (b), rituximab binding to cynomolgus monkey CD40+ B cells (c), and MDX-1342 binding to CHO cells transfected with cynomolgus monkey CD16 (d)
Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.
FC
Figure 6 In vivo B-cell depletion in cynomolgus monkeys: FACS analysis of cynomolgus CD20+ B cells following a single dose of 1 mg/kg MDX-1342 or parental anti-CD19 mAb (a) and cynomolgus CD40+ B cells following a single dose of 0.1 mg/kg MDX-1342, rituximab, or control IgG1 (b)
Cardarelli, P. M., Rao-Naik, C., Chen, S., Huang, H., Pham, A., Moldovan-Loomis, M. C., ... & Kuhne, M. R. (2010). A nonfucosylated human antibody to CD19 with potent B-cell depletive activity for therapy of B-cell malignancies. Cancer immunology, immunotherapy, 59(2), 257.
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Antibody
- Species Reactivity
- Human
- Applications
- FC, ADCC, FuncS
- Related Disease
- Chronic Lymphocytic Leukemia; Rheumatoid Arthritis
Product Property
- Purity
- >90% assessed by SDS-PAGE
- Storage
- Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry, ADCC and Functional Assay.
Target
- Alternative Names
- CD19; CD19 molecule; B4; CVID3; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4
- Gene ID
- 930
- UniProt ID
- P15391
Related Resources
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H17 | Anti-Human CD19 Recombinant Antibody (Denintuzumab Mafodotin) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-1612CL | Human Anti-CD19 Recombinant Antibody (TAB-1612CL) | Depletion, FuncS | Humanized IgG1 |
| TAB-1625CL-S(P) | Anti-Human CD19 Recombinant Antibody scFv Fragment (396) | Depletion, ELISA | Humanized antibody |
| TAB-1625CL-F(E) | Anti-Human CD19 Recombinant Antibody Fab Fragment (396) | Depletion, ELISA | Humanized antibody |
| TAB-431CQ | Anti-Human CD19 Recombinant Antibody | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-108CL, RRID: AB_3111816)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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