CytoStream™ Rabbit Anti-CD19 Recombinant Antibody (VS13-YC154)

Name: CytoStream™ Rabbit Anti-CD19 Recombinant Antibody (VS13-YC154)
SKU: VS13-YC154
Rating: 5 (1 reviews)

CAT#: VS13-YC154

The CytoStream™ Rabbit Anti-CD19 Recombinant Antibody is specific for human CD19. CD19 encodes a molecule engaged in lymphocyte activation, associated with immunodeficiencies affecting pathways like RET signaling critical for cellular differentiation. The anti-CD19 antibody can be used for Western blot (1/1000-1/2000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200), Flow cytometry (1/50-1/1000).

Gene Expression

Normal Tissue Figure 1 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg

Normal Tissue Figure 2 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg

Normal Tissue Figure 3 Appendix

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19

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Specifications

Immunogen
Recombinant human CD19, aa 294-556.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human CD19

Species Reactivity
Human

Applications
WB, ICC, IF, IHC-P, FC

Conjugate
NONE

Related Disease
Common Variable Immunodeficiency

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

Preservative
0.05% Sodium azide

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Shipping with ice pack.

Applications

Application Notes
Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/1000

Target

Alternative Names
CD19 Molecule; B-Lymphocyte Surface Antigen B4; T-Cell Surface Antigen Leu-12; Differentiation Antigen CD19; CD19 Antigen; B-Lymphocyte Antigen CD19; CVID3; B4;

Gene ID
930

UniProt ID
P15391

Long Name
CD19 Molecule

Cellular Localization
Cell membrane

Protein Refseq
XP_011544283.1

Function
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores.

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS13-YC154.pdf

MSDS

MSDS - VS13-YC154.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

CAT
Product Name

Isotype Control

CAT
Product Name

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