What is the immunogen for this CD19 antibody?

The immunogen used is a synthetic peptide comprising the C-terminus of CD19 coupled to keyhole limpet hemocyanin. This recombinant mouse monoclonal antibody specifically reacts with the human CD19 molecule and is validated for use in Immunohistochemistry (IHC).

Is this antibody suitable for use with human samples?

Yes, this antibody specifically reacts with the Human CD19 molecule. It is a mouse IgG1 antibody. CD19 is a marker often used in the study of B cells, and this antibody is suitable for detecting it in human tissue samples.

Mouse Anti-CD19 Recombinant Antibody (clone ME-CD20)

CAT#: MOB-0278MZ

This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human CD19 Molecule.

Gene Expression

Normal Tissue Figure 1 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg

Normal Tissue Figure 2 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg

Normal Tissue Figure 3 Appendix

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19

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Specifications

Immunogen
Synthetic peptide comprising the C-terminus of CD19 coupled to keyhole limpethemocyanin

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human CD19 Molecule

Species Reactivity
Human

Clone
ME-CD20

Applications
IHC

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CD19; Human CD19 Molecule

Gene ID
930

UniProt ID
P15391

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Breast cancer biomarkers at key points during disease progression

Efficient IHC Staining Tool

This recombinant mouse monoclonal antibody worked perfectly for our Immunohistochemistry needs. It specifically reacted with the human CD19 molecule in our tissue samples, providing clear visualization of B-cell distribution without non-specific background staining.

High Purity Reagent

We appreciate the quality of this product. The SDS-PAGE analysis indicated greater than 95 percent purity. It is a reliable clone ME-CD20 that has become a standard reagent in our laboratory for characterizing CD19 expression in various human samples.

Q&As

What is the immunogen for this CD19 antibody?

A: The immunogen used is a synthetic peptide comprising the C-terminus of CD19 coupled to keyhole limpet hemocyanin. This recombinant mouse monoclonal antibody specifically reacts with the human CD19 molecule and is validated for use in Immunohistochemistry (IHC).
Is this antibody suitable for use with human samples?

A: Yes, this antibody specifically reacts with the Human CD19 molecule. It is a mouse IgG1 antibody. CD19 is a marker often used in the study of B cells, and this antibody is suitable for detecting it in human tissue samples.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Primary Immunodeficiency

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-0278MZ.pdf

MSDS

MSDS - MOB-0278MZ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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