Mouse Anti-NHP CD19 Recombinant Antibody (clone PDR134) (CAT#: VS-1024-XY83)
This mouse-derived monoclonal antibody targets CD19, showcasing remarkable reactivity with chimpanzee, human, and non-human primate tissues. The antibody was generated using Pokeweek-stimulated Daudi and Raji cells as the immunogen, ensuring high specificity and efficacy. It is validated for use in Western blot (WB), immunofluorescence (IF), and flow cytometry (FC), making it an invaluable asset for studying CD19 expression and function in a variety of cellular contexts and species.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Colon
(Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg
(Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_7_3.jpg
Figure 2 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_5_6.jpg
Figure 3 Appendix
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_2_2.jpg
Figure 4 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16110/36120_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177455-CD19
Specifications
- Immunogen
- Pokeweek-stimulated Daudi and Raji cells
- Host Species
- Mouse
- Type
- Mouse IgM, kappa
- Specificity
- CD19
- Species Reactivity
- Chimpanzee, Human, Non-human primate
- Clone
- PDR134
- Applications
- WB, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1-2 µg/mL, IF: 1-2 µg/mL, FC: 1-2 µg/106 cells
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H17 | Anti-Human CD19 Recombinant Antibody (Denintuzumab Mafodotin) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-1612CL | Human Anti-CD19 Recombinant Antibody (TAB-1612CL) | Depletion, FuncS | Humanized IgG1 |
| TAB-1625CL | Anti-Human CD19 Recombinant Antibody (396) | Depletion, ELISA | Humanized antibody |
| TAB-1612CL-S(P) | Human Anti-CD19 Recombinant Antibody; scFv Fragment (TAB-1612CL-S(P)) | Depletion, FuncS | Humanized scFv |
| TAB-1625CL-S(P) | Anti-Human CD19 Recombinant Antibody scFv Fragment (396) | Depletion, ELISA | Humanized antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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