Anti-EpCAM Antibody-Cytokine Fusion Protein, GM-CSF-FAB (L19) (CAT#: ACFP-SH049)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EpCAM FAB to GM-CSF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the EpCAM as well as the biological activity of GM-CSF. This fusion protein was directed against EpCAM and induced tumor cell lysis in vitro, which was dependent on the presence of the cytokine. Thus, GM-CSF activated PBMCs to lyse tumor cells, while IL-2 redirected lysis by T cells. This immunocytokine was designed for treating solid tumor.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
Figure 2 Thyroid gland
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
Figure 4 Kidney
(Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
(Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
Specifications
- Target
- EpCAM
- Molecule Class
- GM-CSF-FAB
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- L19
- Related Disease
- Solid tumors
- Cytokine 1
- GM-CSF
- Cytokines 1 Description
- Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called molgramostim.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component; chromosome 4; surface marker (35kD glycoprotein)
- Gene ID
- 4072
- UniProt ID
- P16422
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Biparatopic Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-213 | Humanized Anti-EPCAM Recombinant Antibody (clone Tucotuzumab) | WB, Neut, ELISA, IF, IP, FuncS, FC | Humanized (from mouse) IgG1 |
| TAB-235CL | Human Anti-Human EPCAM Recombinant Antibody (3622W94) | Cyt, FC | Antibody |
| TAB-374MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (h12H8B) | FC, ELISA | Humanized antibody |
| TAB-375MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (h12H8C) | FC, ELISA | Humanized antibody |
| TAB-376MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (h2D11B) | FC, ELISA | Humanized antibody |
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For Research Use Only. Not For Clinical Use.
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