Mouse Anti-EPCAM Recombinant Antibody (clone MOC31) (CAT#: FAMAB-0095YC)
Provided is a mouse monoclonal antibody (MOC31) against the tumor marker EGP-2.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Enrichment by panning.
Cloning of hybridoma MOC31.Enrichment of EGP-2 binding scFv by phage panning.
Krebber, A., Bornhauser, S., Burmester, J., Honegger, A., Willuda, J., Bosshard, H. R., & Plückthun, A. (1997). Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. Journal of immunological methods, 201(1), 35-55.
WB
Figure 2 Immunoblot analysis withanti-EGP-2 MOC31bio of total protein extracts of the following tissues.
This EGP-2 expression pattern was confirmed by Western blotting analysis using the biotinylated EGP-2 specific Mab MOC31.
McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.
IHC
Figure 3 Immunohistochemical analysis of EGP-2 expression in EGP-2 transgenic (tg) BACF2 mice using the biotinylated anti-EGP-2 Mab MOC31bio.
A, crosssection of the lung revealing expression of EGP-2 by the aveolal epithelium (X40) and, C, bronchial epithelium (X25). B, lung of nontransgenic (ntg) littermate (X40). D, human bronchus (X 25). E, cross-section of small intestine in which EGP-2 is strongly expressed by the villus epithelium (X25). F, small intestine of ntg littermate (X25). G, human small intestine (X25). H,cross-section of the tg pancreas. EGP-2 is expressed byboth the exo- and endocrine pancreatic tissue (X40). I,pancreas of ntg littermate (X25). J, cross section of the spleen and part of the small intestine of the EGP-2 tg mouse. The splenic tissue does not express EGP-2; the small intestinal epithelium does (X25).
McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.
Figure 4 Localization study of MOC31bio on i.v. injection in EGP-2 transgenic (tg) and nontransgenic (ntg) mice 3 weeks after B16.F10 and B16.C215 tumor inoculation.
Tumors were isolated 24 h later and analyzed for the presence of biotin by incubation with SA-PO.
McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Clone
- MOC31
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L013E | anti-EPCAM immunotoxin MOC31 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L013D | anti-EPCAM immunotoxin MOC31 (IgG)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L013G | anti-EPCAM immunotoxin MOC31 (IgG)-Gel | Cytotoxicity assay, Functional assay | |
| AGTO-L013R | anti-EPCAM immunotoxin MOC31 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L013O | anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin | Cytotoxicity assay, Functional assay |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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