Mouse Anti-EPCAM Recombinant Antibody (clone MOC31) (CAT#: FAMAB-0095YC)

Provided is a mouse monoclonal antibody (MOC31) against the tumor marker EGP-2.


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  • Gene Expression
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  • COA
ELISA

Figure 1 Enrichment by panning.

Figure 1 Enrichment by panning.

Cloning of hybridoma MOC31.Enrichment of EGP-2 binding scFv by phage panning.

Krebber, A., Bornhauser, S., Burmester, J., Honegger, A., Willuda, J., Bosshard, H. R., & Plückthun, A. (1997). Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. Journal of immunological methods, 201(1), 35-55.

WB

Figure 2 Immunoblot analysis withanti-EGP-2 MOC31bio of total protein extracts of the following tissues.

Figure 2 Immunoblot analysis withanti-EGP-2 MOC31bio of total protein extracts of the following tissues.

This EGP-2 expression pattern was confirmed by Western blotting analysis using the biotinylated EGP-2 specific Mab MOC31.

McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.

IHC

Figure 3 Immunohistochemical analysis of EGP-2 expression in EGP-2 transgenic (tg) BACF2 mice using the biotinylated anti-EGP-2 Mab MOC31bio.

Figure 3 Immunohistochemical analysis of EGP-2 expression in EGP-2 transgenic (tg) BACF2 mice using the biotinylated anti-EGP-2 Mab MOC31bio.

A, crosssection of the lung revealing expression of EGP-2 by the aveolal epithelium (X40) and, C, bronchial epithelium (X25). B, lung of nontransgenic (ntg) littermate (X40). D, human bronchus (X 25). E, cross-section of small intestine in which EGP-2 is strongly expressed by the villus epithelium (X25). F, small intestine of ntg littermate (X25). G, human small intestine (X25). H,cross-section of the tg pancreas. EGP-2 is expressed byboth the exo- and endocrine pancreatic tissue (X40). I,pancreas of ntg littermate (X25). J, cross section of the spleen and part of the small intestine of the EGP-2 tg mouse. The splenic tissue does not express EGP-2; the small intestinal epithelium does (X25).

McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.

Figure 4 Localization study of MOC31bio on i.v. injection in EGP-2 transgenic (tg) and nontransgenic (ntg) mice 3 weeks after B16.F10 and B16.C215 tumor inoculation.

Figure 4 Localization study of MOC31bio on i.v. injection in EGP-2 transgenic (tg) and nontransgenic (ntg) mice 3 weeks after B16.F10 and B16.C215 tumor inoculation.

Tumors were isolated 24 h later and analyzed for the presence of biotin by incubation with SA-PO.

McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Clone
  • MOC31
  • Applications
  • ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone MOC31"

See other products for "EPCAM"

Immunotoxin

CAT Product Name Application Type
AGTO-L013E anti-EPCAM immunotoxin MOC31 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L013D anti-EPCAM immunotoxin MOC31 (IgG)-DT Cytotoxicity assay, Functional assay
AGTO-L013G anti-EPCAM immunotoxin MOC31 (IgG)-Gel Cytotoxicity assay, Functional assay
AGTO-L013R anti-EPCAM immunotoxin MOC31 (IgG)-RTA Cytotoxicity assay, Functional assay
AGTO-L013O anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin Cytotoxicity assay, Functional assay

Humanized Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1163 Hi-Affi™ Rabbit Anti-EPCAM Recombinant Antibody (clone DS1163AB) IHC-P, WB, ICC, ICC, IF Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-106 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Adecatumumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody
AFC-TAB-213 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Tucotuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS ADCC enhanced antibody
AFC-TAB-701 Afuco™ Anti-EpCAM ADCC Recombinant Antibody (Edrecolomab), ADCC Enhanced ELISA, IP, FC, FuncS, IF, Neut ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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