Mouse Anti-EPCAM Recombinant Antibody (clone MOC31) (CAT#: FAMAB-0095YC)
Provided is a mouse monoclonal antibody (MOC31) against the tumor marker EGP-2.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Enrichment by panning.
Cloning of hybridoma MOC31.Enrichment of EGP-2 binding scFv by phage panning.
Krebber, A., Bornhauser, S., Burmester, J., Honegger, A., Willuda, J., Bosshard, H. R., & Plückthun, A. (1997). Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. Journal of immunological methods, 201(1), 35-55.
WB
Figure 2 Immunoblot analysis withanti-EGP-2 MOC31bio of total protein extracts of the following tissues.
This EGP-2 expression pattern was confirmed by Western blotting analysis using the biotinylated EGP-2 specific Mab MOC31.
McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.
IHC
Figure 3 Immunohistochemical analysis of EGP-2 expression in EGP-2 transgenic (tg) BACF2 mice using the biotinylated anti-EGP-2 Mab MOC31bio.
A, crosssection of the lung revealing expression of EGP-2 by the aveolal epithelium (X40) and, C, bronchial epithelium (X25). B, lung of nontransgenic (ntg) littermate (X40). D, human bronchus (X 25). E, cross-section of small intestine in which EGP-2 is strongly expressed by the villus epithelium (X25). F, small intestine of ntg littermate (X25). G, human small intestine (X25). H,cross-section of the tg pancreas. EGP-2 is expressed byboth the exo- and endocrine pancreatic tissue (X40). I,pancreas of ntg littermate (X25). J, cross section of the spleen and part of the small intestine of the EGP-2 tg mouse. The splenic tissue does not express EGP-2; the small intestinal epithelium does (X25).
McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.
Figure 4 Localization study of MOC31bio on i.v. injection in EGP-2 transgenic (tg) and nontransgenic (ntg) mice 3 weeks after B16.F10 and B16.C215 tumor inoculation.
Tumors were isolated 24 h later and analyzed for the presence of biotin by incubation with SA-PO.
McLaughlin, P. M., Harmsen, M. C., Dokter, W. H., Kroesen, B. J., van der Molen, H., Brinker, M. G., ... & de Leij, L. F. (2001). The epithelial glycoprotein 2 (EGP-2) promoter-driven epithelial-specific expression of EGP-2 in transgenic mice: a new model to study carcinoma-directed immunotherapy. Cancer research, 61(10), 4105-4111.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Clone
- MOC31
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L013E | anti-EPCAM immunotoxin MOC31 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L013D | anti-EPCAM immunotoxin MOC31 (IgG)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L013G | anti-EPCAM immunotoxin MOC31 (IgG)-Gel | Cytotoxicity assay, Functional assay | |
| AGTO-L013R | anti-EPCAM immunotoxin MOC31 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L013O | anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin | Cytotoxicity assay, Functional assay |
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