Can this antibody be used for flow cytometry assays?

Absolutely, this humanized recombinant antibody is tailored for use in flow cytometry and cytotoxicity studies. Its high specificity to EPCAM and robust performance in FC applications are supported by consistent production quality and purification methods applied during manufacturing.

What makes this antibody suitable for epithelial cell studies?

The antibody specifically binds to human EPCAM with high affinity due to its humanized design and CHO expression system. Its purity and validated performance in cytotoxicity and FC assays enable accurate detection of EPCAM, supporting detailed research in epithelial cell adhesion processes.

Human Anti-Human EPCAM Recombinant Antibody (3622W94)

CAT#: TAB-235CL

Recombinant Humanized monoclonal antibody expressed in CHO binding to Human EPCAM. 3622W94 is a humanized murine monoclonal antibody (MoAb) against the 17-1A antigen, with potential adjuvant therapeutic properties in colorectal cancer.

Tested Data Published Data Gene Expression

ELISA

Figure 4 Anti-Human EPCAM Recombinant Antibody (TAB-235CL) in ELISA

ELISA analysis of TAB-235CL was performed by coating with human EPCAM protein (His tag).

ELISA

Figure 1 Anti-Human EPCAM Antibody (TAB-235CL) in ELISA

ELISA analysis of TAB-235CL was performed with Human EpCAM Protein, His Tag.
The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 2 Anti-Human EPCAM Antibody (TAB-235CL) in WB

Western blot analysis of TAB-235CL was performed with Human EpCAM Protein, His Tag.
TAB-235CL incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.6μg)

Figure 3 Anti-Human EPCAM Antibody (TAB-235CL) in DB

Dot blot analysis of TAB-235CL was performed with Human EpCAM Protein, His Tag.
TAB-235CL incubation concentration: 2ng/μL.
NC: Negative control PC: Positive control

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Cyt

Figure 1 ADCC activity of five clinically tested anti-EpCAM monoclonal antibodies.

ADCC was determined by dose response analysis as shown for 2 out of 5 human PBMC donors tested.

Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.

Figure 2 Mapping of the binding domain of adecatumumab to exon 5 of human EpCAM.

FACS histograms showing the binding of adecatumumab to 293 cells expressing the EpCAM constructs.

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Subcellular Location Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg

Normal Tissue Figure 3 Colon

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg

Normal Tissue Figure 4 Kidney

Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM

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Specifications

Derivation
Humanized

Type
Antibody

Species Reactivity
Human

Applications
Cyt, FC

Related Disease
Cancer

Product Property

Purity
>95% by HPLC

Storage
Short term: store at 4°C (over 6 months), long term: -20°C or -80°C.

Applications

Application Notes
This antibody has been reported for use in Cytotoxicity and Flow Cytometry.

Target

Alternative Names
EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated an

Gene ID
4072

UniProt ID
P16422

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Breast cancer biomarkers at key points during disease progression

Consistent Performance

I used the humanized anti-EPCAM antibody in flow cytometry and cytotoxicity studies. The antibody has shown consistent performance with excellent specificity to EPCAM, and the high quality of expression in CHO cells supports a robust research output in our cancer model experiments.

High Quality

This anti-EPCAM recombinant antibody exhibits high specificity and purity. It has provided reliable data in cell-based assays such as FC and cytotoxicity evaluations. The antibody's performance in detecting EPCAM makes it a valuable reagent for our laboratory studies related to epithelial cell adhesion research.

Q&As

Can this antibody be used for flow cytometry assays?

A: Absolutely, this humanized recombinant antibody is tailored for use in flow cytometry and cytotoxicity studies. Its high specificity to EPCAM and robust performance in FC applications are supported by consistent production quality and purification methods applied during manufacturing.
What makes this antibody suitable for epithelial cell studies?

A: The antibody specifically binds to human EPCAM with high affinity due to its humanized design and CHO expression system. Its purity and validated performance in cytotoxicity and FC assays enable accurate detection of EPCAM, supporting detailed research in epithelial cell adhesion processes.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-235CL, RRID: AB_3111892)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-235CL.pdf

MSDS

MSDS - TAB-235CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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