Anti-Human EPCAM Recombinant Antibody (Adecatumumab) (CAT#: TAB-106)

Recombinant monoclonal antibody to EpCAM. which is used to target tumor cells. It binds to the epithelial cell adhesion molecule (EpCAM - CD326). Adecatumumab has been used in clinical studies of treatment in prostate and breast cancers.

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FC

Figure 1 Example of FACS binding analysis for ING-1 binding to transfected CHO cells.

Figure 1 Example of FACS binding analysis for ING-1 binding to transfected CHO cells.

FACS histograms are shown with eight CHO cell transfectants.

Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.

FuncS

Figure 2 CDC activity of five clinically tested anti-EpCAM monoclonal antibodies.

Figure 2 CDC activity of five clinically tested anti-EpCAM monoclonal antibodies.

Human serum (10%) was added to cultures of KATO III gastric carcinoma cells and CDC after 45 min monitored by TDA released from lysed cells chelated with Europium and quantified by the fluorescence of the Europium-TDA chelates formed.

Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.

Inhib

Figure 3 The effect of adecatumumab on proliferation of breast cancer cell line and MCF-7 and normal breast epithelia line MCF10A.

Figure 3 The effect of adecatumumab on proliferation of breast cancer cell line and MCF-7 and normal breast epithelia line MCF10A.

The respective cell lines were seeded at 104 cells and cell numbers determined daily for three days. A human IgG1 isotype mAb and a blank served as controls.

Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.

IHC

Figure 4 EpCAM expression by immunohistochemistry on primary/metastatic USPC versus normal endometrial tissue

Figure 4 EpCAM expression by immunohistochemistry on primary/metastatic USPC versus normal endometrial tissue

A representative primary USPC showing strong (3+) EpCAM expression by immunohistochemistry.

El-Sahwi, K., Bellone, S., Cocco, E., Casagrande, F., Bellone, M., Abu-Khalaf, M., ... & Silasi, D. A. (2010). Overexpression of EpCAM in uterine serous papillary carcinoma: implications for EpCAM-specific immunotherapy with human monoclonal antibody adecatumumab (MT201). Molecular cancer therapeutics, 9(1), 57-66.

Cyt

Figure 5 Dose titration cytotoxicity experiments using MT201 against USPC ARK-5.

Figure 5 Dose titration cytotoxicity experiments using MT201 against USPC ARK-5.

ADCC was found to plateau at a dose of 5 to 10 μg/mL in multiple experiments.

El-Sahwi, K., Bellone, S., Cocco, E., Casagrande, F., Bellone, M., Abu-Khalaf, M., ... & Silasi, D. A. (2010). Overexpression of EpCAM in uterine serous papillary carcinoma: implications for EpCAM-specific immunotherapy with human monoclonal antibody adecatumumab (MT201). Molecular cancer therapeutics, 9(1), 57-66.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG1
  • Specificity
  • Tested positive against native human antigen.
  • Species Reactivity
  • Human
  • Applications
  • FC, IHC, FuncS, Inhib, Cyt
  • CAS
  • 503605-66-1
  • Generic Name
  • Adecatumumab
  • MW
  • 147.5 kDa
  • Related Disease
  • Breast cancers

Product Property

  • Purity
  • >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Storage
  • Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.

Applications

  • Application Notes
  • The EPCAM antibody has been reported in applications of Inhibition, Flow Cytometry, Immunohistochemistry, Functional Assay, Cytoxicity.

Target

  • Alternative Names
  • Adecatumumab;503605-66-1;MT201;EPCAM;epithelial cell adhesion molecule;antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1;17 1A;323/A3;CD326;CO 17A;EGP 2;EGP34;EGP40;Ep CAM;ESA;GA733 2;HEA125;KS1/4;K

Related Resources

  • Biosimilar Overview
Please refer to Adecatumumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Adecatumumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Adecatumumab"

Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Adecatumumab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to EpCAM. which is used to target tumor cells. It binds to the epithelial cell adhesion molecule (EpCAM - CD326). Adecatumumab has been used in clinical studies of treatment in prostate and breast cancers.

See other products for "EPCAM"

Humanized Antibody

CAT Product Name Application Type
TAB-112-F(E) Anti-Human EpCAM Recombinant Antibody Fab Fragment, 17-1A (Citatuzumab Bogatox) Neut, ELISA, IF, IP, FuncS, FC, ICC Fab - G1 - kappa
TAB-374MZ Anti-Human EPCAM Recombinant Antibody (h12H8B) FC, ELISA Humanized antibody
TAB-375MZ Anti-Human EPCAM Recombinant Antibody (h12H8C) FuncS, Inhib Humanized antibody
TAB-376MZ Anti-Human EPCAM Recombinant Antibody (h2D11B) Cyt, Inhib Humanized antibody
TAB-362MZ-S(P) Anti-Human EPCAM Recombinant Antibody scFv Fragment (3622W94) Confocal, ELISA Humanized antibody

Immunotoxin

CAT Product Name Application Type
AGTO-L013E anti-EPCAM immunotoxin MOC31 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L013D anti-EPCAM immunotoxin MOC31 (IgG)-DT Cytotoxicity assay, Functional assay
AGTO-L013G anti-EPCAM immunotoxin MOC31 (IgG)-Gel Cytotoxicity assay, Functional assay
AGTO-L013R anti-EPCAM immunotoxin MOC31 (IgG)-RTA Cytotoxicity assay, Functional assay
AGTO-L013O anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin Cytotoxicity assay, Functional assay

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1163 Hi-Affi™ Rabbit Anti-EPCAM Recombinant Antibody (clone DS1163AB) IHC-P, WB, ICC, ICC, IF Rabbit IgG

Recombinant Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-106 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Adecatumumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody
AFC-TAB-213 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Tucotuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS ADCC enhanced antibody
AFC-TAB-701 Afuco™ Anti-EpCAM ADCC Recombinant Antibody (Edrecolomab), ADCC Enhanced ELISA, IP, FC, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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