Anti-Human EPCAM Recombinant Antibody (3-171) (CAT#: TAB-356MZ)

This antibody 3-171 that binds to Epithelial Cell Adhesion Molecule (EpCAM) shows good affinity, good cross-reactivity profiles and excellent ADCC and CDCC activity, which can be potentially used in the treatment studies of cancer.

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FC

Figure 1 shows flow cytometric analysis of 3-171 IgG, MOC31 IgG and MT201 IgG binding to the naturally EpCAM+ Kato III cell line.

Figure 1 shows flow cytometric analysis of 3-171 IgG, MOC31 IgG and MT201 IgG binding to the naturally EpCAM+ Kato III cell line.

Anti-green fluorescent protein (GFP) antibody is used as a negative control. MFI= Median Fluorescence Intensity.

WB

Figure 2 Western blot binding analysis of 3-171 IgG binding to human (Hu) and Cynomolgus (Cy) EpCAM-Fc variants under non-reducing and reducing conditions. M= Molecular weight markers.

Figure 2 Western blot binding analysis of 3-171 IgG binding to human (Hu) and Cynomolgus (Cy) EpCAM-Fc variants under non-reducing and reducing conditions. M= Molecular weight markers.

ELISA

Figure 3 shows ELISA binding analysis of 3-171 IgG to human and cynomolgus EpCAM-Fc variants.

Figure 3 shows ELISA binding analysis of 3-171 IgG to human and cynomolgus EpCAM-Fc variants.

MAXI-Sorb plates were coated with human and cynomolgus EpCAM-Fc at a concentration of 1 μg/ml. The antibody was added in 2-fold dilutions from 133 nM to 32 fM.

Cyt

Figure 4 shows that 3-171 IgG induces ADCC in MDA-MB-453 cells in the presence of human PBMCs.

Figure 4 shows that 3-171 IgG induces ADCC in MDA-MB-453 cells in the presence of human PBMCs.

These data demonstrate that 3-171 Ig possesses superior ADCC activity over the positive control antibody, MT201 IgG.

ELISA

Figure 5 shows ELISA binding analysis of 3-17I scFv, 7-F17 scFv, 17- C20 scFv and 24-G6 scFv to human EpCAM-Fc.

Figure 5 shows ELISA binding analysis of 3-17I scFv, 7-F17 scFv, 17- C20 scFv and 24-G6 scFv to human EpCAM-Fc.

MAXI-Sorb plates were coated with human or cynomolgus EpCAM-Fc at a concentration of 5 μg/ml. The antibody was added in 2-fold dilutions from 667 nM to 3.2 nM.


Specifications

  • Host Species
  • Human
  • Type
  • Human antibody
  • Specificity
  • Human
  • Species Reactivity
  • Monkey
  • Clone
  • 3-171
  • Applications
  • Cyt, ELISA, FC, WB
  • Related Disease
  • Cancers such as breast, colorectal, prostate, ovary, bladder, gallbladder, pancreas, lung cancer

Applications

  • Application Notes
  • This antibody has been reported for use in Cytotoxicity, Enzyme-linked Immunosorbent Assay, Flow Cytotoxicityometry, Western Blot.

Target

  • Alternative Names
  • EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein)

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 3-171"

See other products for "EPCAM"

Immunotoxin

CAT Product Name Application Type
AGTO-L013E anti-EPCAM immunotoxin MOC31 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L013D anti-EPCAM immunotoxin MOC31 (IgG)-DT Cytotoxicity assay, Functional assay
AGTO-L013G anti-EPCAM immunotoxin MOC31 (IgG)-Gel Cytotoxicity assay, Functional assay
AGTO-L013R anti-EPCAM immunotoxin MOC31 (IgG)-RTA Cytotoxicity assay, Functional assay
AGTO-L013O anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin Cytotoxicity assay, Functional assay

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1163 Hi-Affi™ Rabbit Anti-EPCAM Recombinant Antibody (clone DS1163AB) IHC-P, WB, ICC, ICC, IF Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-106 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Adecatumumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody
AFC-TAB-213 Afuco™ Anti-EPCAM ADCC Recombinant Antibody (Tucotuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS ADCC enhanced antibody
AFC-TAB-701 Afuco™ Anti-EpCAM ADCC Recombinant Antibody (Edrecolomab), ADCC Enhanced ELISA, IP, FC, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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