Anti-Human EPCAM Recombinant Antibody (3-171) (CAT#: TAB-356MZ)
This antibody 3-171 that binds to Epithelial Cell Adhesion Molecule (EpCAM) shows good affinity, good cross-reactivity profiles and excellent ADCC and CDCC activity, which can be potentially used in the treatment studies of cancer.
Specific Inquiry
FC
Figure 1 shows flow cytometric analysis of 3-171 IgG, MOC31 IgG and MT201 IgG binding to the naturally EpCAM+ Kato III cell line.
Anti-green fluorescent protein (GFP) antibody is used as a negative control. MFI= Median Fluorescence Intensity.
WB
Figure 2 Western blot binding analysis of 3-171 IgG binding to human (Hu) and Cynomolgus (Cy) EpCAM-Fc variants under non-reducing and reducing conditions. M= Molecular weight markers.
ELISA
Figure 3 shows ELISA binding analysis of 3-171 IgG to human and cynomolgus EpCAM-Fc variants.
MAXI-Sorb plates were coated with human and cynomolgus EpCAM-Fc at a concentration of 1 μg/ml. The antibody was added in 2-fold dilutions from 133 nM to 32 fM.
Cyt
Figure 4 shows that 3-171 IgG induces ADCC in MDA-MB-453 cells in the presence of human PBMCs.
These data demonstrate that 3-171 Ig possesses superior ADCC activity over the positive control antibody, MT201 IgG.
ELISA
Figure 5 shows ELISA binding analysis of 3-17I scFv, 7-F17 scFv, 17- C20 scFv and 24-G6 scFv to human EpCAM-Fc.
MAXI-Sorb plates were coated with human or cynomolgus EpCAM-Fc at a concentration of 5 μg/ml. The antibody was added in 2-fold dilutions from 667 nM to 3.2 nM.
Specifications
- Host Species
- Human
- Type
- Human antibody
- Specificity
- Human
- Species Reactivity
- Monkey
- Clone
- 3-171
- Applications
- Cyt, ELISA, FC, WB
- Related Disease
- Cancers such as breast, colorectal, prostate, ovary, bladder, gallbladder, pancreas, lung cancer
Applications
- Application Notes
- This antibody has been reported for use in Cytotoxicity, Enzyme-linked Immunosorbent Assay, Flow Cytotoxicityometry, Western Blot.
Target
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein)
- Gene ID
- 4072
- UniProt ID
- P16422
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Immunotoxin
Humanized Antibody
Mouse Antibody
Human Antibody
Recombinant Antibody
Rabbit Monoclonal Antibody
Fab Fragment Antibody
ADCC Enhanced Antibody
MHC Tetramer for Cancer
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L013E | anti-EPCAM immunotoxin MOC31 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L013D | anti-EPCAM immunotoxin MOC31 (IgG)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L013G | anti-EPCAM immunotoxin MOC31 (IgG)-Gel | Cytotoxicity assay, Functional assay | |
| AGTO-L013R | anti-EPCAM immunotoxin MOC31 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L013O | anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin | Cytotoxicity assay, Functional assay |
Customer Reviews and Q&As
Customer Review
Q&As
-
Long Shelf-Life
The long shelf-life of this antibody is a huge plus. It maintains its efficacy even after several months of storage, which is important for long-term projects. -
Reliable Purification
The antibody is easy to purify and handle. Using affinity columns, I achieved high-purity fractions, which is crucial for downstream applications. -
Impressive Performance
I am impressed with the performance of this antibody in my immunoassays. It delivers clear, specific results and has improved the quality of my research.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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