anti-EPCAM immunotoxin MOC31 (IgG)-PE
CAT#: AGTO-L013E
This immunotoxin was achieved by conjugating/fusing the Anti-EPCAM monoclonal antibody to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to carcinoma cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of cancers.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
❮
❯
Specifications
- Host Species
- Mouse
- Cell Penetrating Peptide
- P16422
- Species Reactivity
- Human
- Clone
- MOC31
- Molecule Class
- L kappa; IgG1-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Hepatocellular carcinomas (HCC)
Target
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Hepatocellular carcinomas; HCC; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35
- Gene ID
- 4072
- UniProt ID
- P16422
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Conjugation Success
This antibody has been effectively conjugated with several labels for use in a variety of experimental applications, and its binding properties have not changed.
Suitable for Various Applications
The versatility of this antibody is commendable. We have used it in multiple applications, and it has not disappointed us in any.
Stable for Long-Term Use
We stored the antibody as recommended and found it to be stable and functional even after several months. It's excellent for long-term projects.
Q&As
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How do I perform antibody titration for my experiment?
A: To titrate this antibody, prepare a serial dilution series, and test each dilution in your assay. This will help you determine the optimal concentration that provides the best signal-to-noise ratio.
-
Are there special handling instructions for this antibody?
A: Handle the antibody with care, avoiding contamination. Use sterile techniques, aliquot to avoid multiple freeze-thaw cycles, and follow storage instructions closely to maintain efficacy.
-
Is it necessary to include a blocking step in my protocol?
A: Yes, including a blocking step using agents like BSA or non-fat dry milk is essential to reduce non-specific binding and background noise, thereby improving the assay specificity.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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