Anti-EpCAM Immunocytokine, scFv-CH1 (3B10)-GM-CSF: scFv-Cκ (3B10)-IL2 (ACFP-SH193)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EpCAM scFv-CH1; scFv-Cκ to IL2 and GM-CSF. It was expressed in CHO and purified with affinity chromatography. The Immunocytokines retains the ability to bind the EpCAM as well as the biological activity of IL2 and GM-CSF. These bi-antibody-cytokine fusion proteins exhibited the ability to bind the targeted antigen and retained the biological activity of murine IL-2 and GM-CSF to induce CTLL-2 and FDC-P1 cell proliferation respectively. The mDCH-3B10 fusion protein, tested by a systemic treatment with the bi-antibody-cytokine fusion protein, induced significant inhibition of pulmonary metastases of mouse colon carcinoma CT26 expressing human KSA (CT26-KSA) cells in immunocompetent BALB/c mice. This Immunocytokines was designed for treating EpCAM over-expressing cancers.
Specifications
- Target
- EpCAM
- Molecule Class
- scFv-CH1-GM-CSF; scFv-Cκ-IL2
- Species Reactivity
- Human
- Host
- Mouse
- Expression Host
- CHO
- Clone
- 3B10
- Related Disease
- EpCAM over-expressing cancers
- Cytokines 1
- IL2
- Cytokines 1 Description
- Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
- Cytokine 1 Species
- Mouse
- Cytokines 2
- GM-CSF
- Cytokines 2 Description
- Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
- Cytokine 2 Species
- Mouse
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2
- Entrez Gene ID
- 4072
- UniProt ID
- P16422
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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