Anti-Human MUC1 Recombinant Antibody (H23) (CAT#: TAB-426MZ)

Figure 1 Binding of MUC1-expressing cells and tumor sections by humanized H23.

Flow-cytometry analysis. Mouse DA3 transfected cells, expressing the MUC1 gene were incubated with 100 μg/ml HumH23 MBP-scFv (I), 100 μg/ml MuH23 MBP-scFv (II), 10 μg/ml of H23 Mab (III), 100 μg/ml anti-fluorescein MBP-4-4-20(Fv) (IV), for 2 h at room temperature. The cells that were incubated with MBP-scFvs were stained with a mouse anti-MBP Mab followed by FITC-labeled goat anti-mouse IgG antibodies. Cells that were incubated with H23 Mab were stained with FITC-labeled goat anti-mouse IgG antibodies. As control, cells were stained with FITC-labeled secondary antibodies alone.

Mazor, Y., Keydar, I., & Benhar, I. (2005). Humanization and epitope mapping of the H23 anti-MUC1 monoclonal antibody reveals a dual epitope specificity. Molecular immunology, 42(1), 55-69.

Figure 2 Binding of MUC1-expressing cells and tumor sections by humanized H23.

Immunohistochemical staining of breast cancer cells and tumor sections. Paraffin-embedded T47D breast cancer cells (I–III with 63× magnification) and breast cancer tissue samples (IV–VI with 40× magnification) were stained with 100 μg/ml HumH23 MBP-scFv (I, IV), anti-fluorescein MBP-4-4-20(Fv) (II, V) or 10 μg/ml of H23 Mab (III,VI). MBP-scFvs were detected with anti-MBP Mab followed by HRP-conjugated goat anti-mouse antibodies that were also used to detect staining by the H23 Mab.

Mazor, Y., Keydar, I., & Benhar, I. (2005). Humanization and epitope mapping of the H23 anti-MUC1 monoclonal antibody reveals a dual epitope specificity. Molecular immunology, 42(1), 55-69.