Recombinant Rabbit Anti-PLCG1 Antibody (clone R01-6B4) (CAT#: VS3-FY1149)
This product is a recombinant rabbit antibody that recognizes PLCG1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-6B4 is specific for human, rat PLCG1. The antigen is synthetic peptide of human phospholipase c gamma 1/plc-gamma-1.
Specific Inquiry
WB
Figure 1. Recombinant Rabbit Anti-PLCG1 Antibody (clone R01-6B4) in WB.
Western Blot analysis of Phospho-lipase C gamma 1/PLCgamma1 in U2OS, C6 lysates using Phospho-lipase C gamma 1/PLCgamma1 Antibody.
Specifications
- Immunogen
- Synthetic peptide of human phospholipase C gamma 1/PLC-gamma-1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat PLCG1
- Species Reactivity
- Human, Rat
- Clone
- R01-6B4
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 149 kDa; Observed MW: 149 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PLCG1; PLC1; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1
- Gene ID
- 5335
- UniProt ID
- P19174
- Cellular Localization
- Cell projection
- Post Translation Modifications
- Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA (By similarity).
Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells.
Ubiquitinated by CBLB in activated T-cells.
- Protein Refseq
- NP_002651.2; NP_877963.1
- Function
- Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity).
Plays a role in actin reorganization and cell migration (PubMed:17229814).
Recommended Products
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1663z | Mouse Anti-PLCG1 Recombinant Antibody (clone 26H9) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-0171CT | Recombinant Mouse anti-Human PLCG1 Monoclonal antibody (EML671) | WB | |
| FAMAB-0249YC | Mouse Anti-PLCG1 Recombinant Antibody (clone F7) | ELISA, IP, WB | Mouse IgG |
| VS3-CJ744 | Rabbit Anti-PLCG1 Recombinant Antibody (VS3-CJ744) | WB, ICC, IP | Rabbit IgG |
| VS3-FY2113 | Rabbit Anti-PLCG1 Recombinant Antibody (clone R81-6X-8) | WB, IF, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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