Recombinant Mouse Anti-Prnp Antibody (143)
CAT#: EPAF-1417LC
This product is a mouse monoclonal antibody that specifically recognizes DWEDRYYRE, which is an linear epitope on Major prion protein from Mouse. The Prnp-binding antibody 143 is an epitope-specific antibody that can be used in immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
❮
❯
Specifications
- Host Species
- Mouse
- Specificity
- Mouse Major prion protein
- Species Reactivity
- Mouse
- Clone
- 143
- Applications
- Immunofluorescence
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| FAMAB-0272CQ-S(P) | Mouse Anti-PRNP Recombinant Antibody (clone ch31C6); scFv Fragment | ELISA, WB, IHC | Mouse scFv |
| FAMAB-0273CQ-S(P) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 106); scFv Fragment | ELISA, WB, IHC | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| MRO-948LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (6H10) | ELISA | Mouse antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (D13) | ELISA | Human antibody |
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| MRO-946LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (R1) | ELISA | Human antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0594CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM68) | ELISA | IgG |
| EPAF-0595CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM39) | ELISA | IgG |
| EPAF-0596CQ | Recombinant Mouse Anti-Human PRNP Antibody (ION6) | ELISA | IgG |
| EPAF-0597CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM56) | ELISA | IgG1 |
| EPAF-0598CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM72) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0294-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone D18); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0295-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 3:3); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0296-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:4); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0297-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:6); Fab Fragment | WB, IF, IHC | Human Fab |
| HPAB-0909-FY-S(P) | Mouse Anti-PRNP Recombinant Antibody scFv Fragment (HPAB-0909-FY-S(P)) | WB, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY84 | Human Anti-PRNP (clone mAb132) scFv-Fc Chimera | ELISA, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1763 | Anti-PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5727 | Anti-Human PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5728 | Anti-Mouse PRNP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC150 | Recombinant Anti-PRNP (AA 56-90 x AA 83-89) Biparatopic Antibody, Tandem scFv (mAb 106 x mAb 110) | ELISA, IHC, WB | Tandem scFv |
| VS-0525-YC152 | Recombinant Anti-PRNP (AA 119-127 x AA 143-149) Biparatopic Antibody, Tandem scFv (mAb 132 x Clone 31C6) | ELISA, IHC, WB | Tandem scFv |
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