Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC39)
CAT#: VS-1025-YC39
The Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC39) is an antibody prodrug comprised of a recombinant human antibody targeting human CD274, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to CD274 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to CD274 effectively. CD274, or PD-L1, is an immune checkpoint molecule frequently expressed by tumor cells to achieve immune evasion. By binding to the PD-1 receptor on T-cells, it delivers an inhibitory signal, effectively stopping the T-cell from recognizing and killing the cancer. Blockade of the PD-1/PD-L1 axis with antibodies is a cornerstone of modern immunotherapy.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Normal Tissue
Figure 5 Placenta
Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human CD274
- Target
- CD274
- Species Reactivity
- Human
- Isotype
- IgG4, kappa
- Epitope
- CD274-ECD
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
- Alternative Names
- B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
- Gene ID
- 29126
- UniProt ID
- Q9NZQ7
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Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-305-sdAb | Recombinant Anti-Mouse Cd274 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| HPAB-N0408-YC-VHH | Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0408-YC-VHH) | ELISA, FC, BL | Human VHH |
| HPAB-N0307-YC-VHH | Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH) | ELISA, FC, BL | Humanized VHH |
| NBD-0065-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH) | ELISA | Camelid VHH |
| NBD-0066-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH) | ELISA, Inihb | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1814z | Mouse Anti-CD274 Recombinant Antibody (clone 7A5) | FC, IHC, FuncS | Mouse IgG2b |
| PABL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006) | WB, ELISA, FuncS | Human IgG |
| MOB-2354MZ | Mouse Anti-CD274 Recombinant Antibody (clone NJI3) | FC | Mouse IgG1 |
| HPAB-0193-YC | Human Anti-CD274 Recombinant Antibody (HPAB-0193-YC) | ELISA | Humanized IgG4, κ |
| HPAB-0194-YC | Mouse Anti-CD274 Recombinant Antibody (HPAB-0194-YC) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-0193-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0193-YC-S(P)) | ELISA | Human scFv |
| HPAB-0194-YC-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0194-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0195-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0195-YC-S(P)) | SPR | Humanized scFv |
| HPAB-0196-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0196-YC-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| HPAB-0193-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E)) | ELISA | Humanized Fab |
| HPAB-0194-YC-F(E) | Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0195-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E)) | SPR | Humanized Fab |
| HPAB-0196-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0190CL | Anti-Human CD274 Recombinant Antibody (PD1-AB1) | FC, ELISA, FuncS | |
| TAB-0997CL | Anti-Human CD274 Recombinant Antibody (B60-55) | ELISA, FuncS, FC, PK | Human antibody |
| TAB-1002CL | Mouse Anti-CD274 Recombinant Antibody (TAB-1002CL) | ELISA, FC | Mouse IgG, κ |
| TAB-1003CL | Human Anti-CD274 Recombinant Antibody (TAB-1003CL) | FC, ELISA | Human IgG |
| TAB-1004CL | Human Anti-CD274 Recombinant Antibody (TAB-1004CL) | FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0262CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Mouse IgG |
| TAB-0992CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL) | Inhib, FC, SPR | Mouse IgG |
| TAB-0993CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0993CL) | FC, SPR | Mouse IgG |
| TAB-0994CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0994CL) | ELISA, FC | Mouse IgG |
| TAB-1009CL | Mouse Anti-CD274 Recombinant Antibody (TAB-1009CL) | WB, IHC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0263CL | Human Anti-CD274 Recombinant Antibody (TAB-0263CL) | ELISA, WB, SPR, FuncS | Humanized IgG |
| TAB-0264CL | Human Anti-CD274 Recombinant Antibody (TAB-0264CL) | ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib | Humanized IgG |
| TAB-0265CL | Human Anti-CD274 Recombinant Antibody (TAB-0265CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
| TAB-0266CL | Human Anti-CD274 Recombinant Antibody (TAB-0266CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
| TAB-0267CL | Human Anti-CD274 Recombinant Antibody (TAB-0267CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0995CL | Human Anti-CD274 Recombinant Antibody (TAB-0995CL) | ELISA, FC | Chimeric (Mouse/Human) IgG1, κ |
| TAB-0995CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0995CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-324CQ | Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6) | IHC-P, FC, Neut, ICC | Mouse IgG2b, κ |
| NEUT-325CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-206) | Neut | Human IgG1, κ |
| NEUT-326CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-207) | ELISA, Neut | Human IgG1, κ |
| NEUT-327CQ | Mouse Anti-CD274 Recombinant Antibody (clone MIH1) | FC, FuncS, Neut, IHC, IF | Mouse IgG1, κ |
| NEUT-330CQ | Rat Anti-CD274 Recombinant Antibody (clone MIH5) | FC, FuncS, Neut, IHC-Fr, WB | Rat IgG2a, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-328CQ | Rat Anti-CD274 Recombinant Antibody (VMC171) | Block, FC | Rat IgG2 |
| NEUT-329CQ | Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2) | FC, IF, Block | Rat IgG2b, κ |
| NEUT-331CQ | Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1) | ELISA, Block | Mouse IgG1, λ |
| NEUT-332CQ | Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009) | Block | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0548 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB) | ELISA | Rabbit IgG |
| MOR-4049 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS) | ELISA | Rabbit IgG |
| MOR-4050 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS) | ICC, IF, FC | Rabbit IgG |
| MOR-4051 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-417CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| AFC-TAB-418CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ) | ELISA, IHC, FC, IP, IF, BL | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC110 | Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced | ELISA, WB, SPR, ADCC, CDC | Antibody hexamer |
| VS-0924-YC111 | Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced | ELISA, WB, SPR, ADCC, CDC, Inhib | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY375 | Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY32 | CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY65 | Human Anti-CD274 (clone MI4736) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC608 | Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608) | Block, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1152 | Anti-CD274 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1153 | Anti-Mouse CD274 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1154 | Anti-Human CD274 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC202 | Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv | ELISA, IHC, FC, IP, IF | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC8 | SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC8) | ELISA | Mouse IgG |
| VS-0825-YC47 | SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC47) | ELISA, IHC, FC, IP, IF, Inhib | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC9 | Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC9) | ISZ, Cyt, FuncS |
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