Human Anti-CD274 (clone MI4736) scFv-Fc Chimera (CAT#: VS-0325-FY65)
The anti-CD274 scFv-Fc antibody is a specialized therapeutic and diagnostic molecule designed to target the CD274 protein, also known as PD-L1 (Programmed Death-Ligand 1). This protein is involved in the immune checkpoint pathway, which cancer cells often exploit to evade immune detection and destruction. The development of anti-CD274 antibodies aims to enhance anti-tumor immunity, making them a promising avenue for cancer treatment.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Figure 4 Colon
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
(Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Figure 5 Placenta
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
(Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
Specifications
- Immunogen
- The human PDL1 protein.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human CD274
- Species Reactivity
- Human
- Clone
- MI4736
- Applications
- ELISA, Inhibition
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1
- Gene ID
- 29126
- UniProt ID
- Q9NZQ7
- Long Name
- CD274 Molecule
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Cellular Localization
- Cell membrane; Endosome; Membrane; Nucleus; Secreted
- Post Translation Modifications
- Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.
Ubiquitinated by MARCHF8; leading to degradation.
Glycosylation at Asn35, Asn192, Asn200, and Asn219
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 5 sites
- Protein Refseq
- XP_047279218.1; XP_054218785.1; NP_001254635.1; NP_001300958.1; NP_054862.1
- Function
- Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410).
As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0263CL | Human Anti-CD274 Recombinant Antibody (TAB-0263CL) | ELISA, WB, SPR, FuncS | Humanized IgG |
| TAB-0264CL | Human Anti-CD274 Recombinant Antibody (TAB-0264CL) | ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib | Humanized IgG |
| TAB-0996CL | Human Anti-CD274 Recombinant Antibody (TAB-0996CL) | ELISA, FC | Human IgG1, κ |
| TAB-0996CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0996CL-F(E)) | ELISA, FC | Humanized Fab |
| TAB-418CQ | Anti-Human CD274 Recombinant Antibody (TAB-418CQ) | ELISA, IHC, FC, IP, IF, BL | IgG1, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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