Human Anti-CD274 (clone MI4736) scFv-Fc Chimera

Name: Human Anti-CD274 (clone MI4736) scFv-Fc Chimera
SKU: VS-0325-FY65
Rating: 5 (1 reviews)

CAT#: VS-0325-FY65

The anti-CD274 scFv-Fc antibody is a specialized therapeutic and diagnostic molecule designed to target the CD274 protein, also known as PD-L1 (Programmed Death-Ligand 1). This protein is involved in the immune checkpoint pathway, which cancer cells often exploit to evade immune detection and destruction. The development of anti-CD274 antibodies aims to enhance anti-tumor immunity, making them a promising avenue for cancer treatment.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

❮

❯

Specifications

Immunogen
The human PDL1 protein.

Host Species
Human

Type
Human IgG1, scFv-Fc

Specificity
Human CD274

Species Reactivity
Human

Clone
MI4736

Applications
ELISA, Inhibition

Related Disease
Cancer

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Target

Alternative Names
CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1

Gene ID
29126

UniProt ID
Q9NZQ7

Long Name
CD274 Molecule

Sequence Similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.

Cellular Localization
Cell membrane; Endosome; Membrane; Nucleus; Secreted

Post Translation Modifications
Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.
Ubiquitinated by MARCHF8; leading to degradation.
Glycosylation at Asn35, Asn192, Asn200, and Asn219
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 5 sites

Protein Refseq
XP_047279218.1; XP_054218785.1; NP_001254635.1; NP_001300958.1; NP_054862.1

Function
Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410).
As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response.

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for VS-0325-FY65. Click the button above to contact us or submit your feedback about this product.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0325-FY65.pdf

MSDS

MSDS - VS-0325-FY65.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

See other products for "Clone MI4736"

CAT
Product Name

See other products for "Cd274"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
NAB-305-sdAb	Recombinant Anti-Mouse Cd274 VHH Single Domain Antibody	IHC, IP, FC, Neut, FUNC	Llama VHH
HPAB-N0307-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH)	ELISA, FC, BL	Humanized VHH
NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH
NBD-0066-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH)	ELISA, Inihb	Camelid VHH
NBD-0067-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0067-YJ-VHH)	ELISA, FC	Camelid VHH

CAT	Product Name	Application	Type
PFBL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006); Fab Fragment	WB, ELISA, FuncS	Human Fab
HPAB-0193-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E))	ELISA	Humanized Fab
HPAB-0194-YC-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E))	ELISA	Mouse Fab
HPAB-0195-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E))	SPR	Humanized Fab
HPAB-0196-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E))	FC	Human Fab

CAT	Product Name	Application	Type
TAB-0992CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL)	Inhib, FC, SPR	Mouse IgG
TAB-0993CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0993CL)	FC, SPR	Mouse IgG
TAB-0994CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0994CL)	ELISA, FC	Mouse IgG
TAB-1009CL	Mouse Anti-CD274 Recombinant Antibody (TAB-1009CL)	WB, IHC	Mouse IgG1, κ
TAB-1010CL	Anti-Human CD274 Recombinant Antibody	FC

CAT	Product Name	Application	Type
TAB-0995CL	Human Anti-CD274 Recombinant Antibody (TAB-0995CL)	ELISA, FC	Chimeric (Mouse/Human) IgG1, κ
TAB-0995CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P))	ELISA, FC	Human scFv
TAB-0995CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E))	ELISA, FC	Chimeric (Mouse/Human) Fab

CAT	Product Name	Application	Type
TAB-0263CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0263CL-S(P))	ELISA, WB, SPR, FuncS	Humanized scFv
TAB-0264CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0264CL-S(P))	ELISA, WB, SPR, Inhib, FuncS	Humanized scFv
TAB-0265CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0265CL-S(P))	ELISA, WB, SPR, FuncS	Humanized scFv
TAB-0266CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0266CL-S(P))	ELISA, WB, SPR, FuncS	Humanized scFv
TAB-0267CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0267CL-S(P))	ELISA, WB, SPR, FuncS	Humanized scFv

CAT	Product Name	Application	Type
TAB-1003CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1003CL-F(E))	FC, ELISA	Human Fab
TAB-1004CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1004CL-F(E))	FC	Human Fab
TAB-1005CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1005CL-F(E))	FC	Human Fab
TAB-1006CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1006CL-F(E))	FC	Human Fab
TAB-1007CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1007CL-F(E))	FC	Human Fab

CAT	Product Name	Application	Type
NEUT-324CQ	Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6)	IHC-P, FC, Neut, ICC	Mouse IgG2b, κ
NEUT-325CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-206)	Neut	Human IgG1, κ
NEUT-326CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-207)	ELISA, Neut	Human IgG1, κ
NEUT-327CQ	Mouse Anti-CD274 Recombinant Antibody (clone MIH1)	FC, FuncS, Neut, IHC, IF	Mouse IgG1, κ
NEUT-330CQ	Rat Anti-CD274 Recombinant Antibody (clone MIH5)	FC, FuncS, Neut, IHC-Fr, WB	Rat IgG2a, λ

CAT	Product Name	Application	Type
NEUT-328CQ	Rat Anti-CD274 Recombinant Antibody (VMC171)	Block, FC	Rat IgG2
NEUT-329CQ	Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2)	FC, IF, Block	Rat IgG2b, κ
NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ
NEUT-332CQ	Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009)	Block	Rabbit IgG

CAT	Product Name	Application	Type
MOR-0548	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB)	ELISA	Rabbit IgG
MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG
MOR-4050	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS)	ICC, IF, FC	Rabbit IgG
MOR-4051	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS)	FC	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-N0326-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-N0326-YC-S(P))	ELISA, FuncS	Human scFv
HPAB-0528LY-S(P)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-1); scfv Fragment	ELISA	Human scfv
HPAB-0529LY-S(P)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-2); scfv Fragment	ELISA	Human scfv
HPAB-0530LY-S(P)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-3); scfv Fragment	ELISA	Human scfv
HPAB-0531LY-S(P)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-4); scfv Fragment	ELISA	Human scfv

CAT	Product Name	Application	Type
HPAB-0046LY	Human Anti-CD274 Recombinant Antibody (HPAB-0046LY)	WB	Human IgG4
HPAB-0561LY	Human Anti-CD274 Recombinant Antibody (HPAB-0561LY)	ELISA, Block	Chimeric (mouse/human) IgG1
HPAB-0562LY	Human Anti-CD274 Recombinant Antibody (HPAB-0562LY)	ELISA, Block	Chimeric (mouse/human) IgG1
HPAB-0563LY	Human Anti-CD274 Recombinant Antibody (HPAB-0563LY)	ELISA, Block	Chimeric (mouse/human) IgG1
HPAB-0564LY	Human Anti-CD274 Recombinant Antibody (HPAB-0564LY)	ELISA, Block	Chimeric (mouse/human) IgG1

CAT	Product Name	Application	Type
AFC-TAB-417CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody
AFC-TAB-418CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer
VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

CAT	Product Name	Application	Type
VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application
VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC
VS-0525-XY1153	Anti-Mouse CD274 Immunohistochemistry Kit	IHC
VS-0525-XY1154	Anti-Human CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 3 Anti-Human TNF beta Antibody (TAB-071) in DB

Anti-Human TNF beta Recombinant Antibody (TAB-071) (CAT# TAB-071)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Figure 7 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in ELISA

Human Anti-NECTIN4 Recombinant Antibody (TAB-H25) (CAT# TAB-H25)
Application: ELISA, Neut, IF, IP, FC, FuncS

Figure 2 Anti-SLAMF7 Recombinant Antibody (TAB-743) in WB

Anti-SLAMF7 Recombinant Antibody (Elotuzumab) (CAT# TAB-743)
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC

Figure 2 Anti-Human CD3 Antibody (TAB-159) in FC

Anti-Human CD3 Recombinant Antibody (TAB-159) (CAT# TAB-159)
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

Figure 2 Anti-Human EGFR Antibody (PABW-088) in DB

Mouse Anti-EGFR Recombinant Antibody (clone 7A7) (CAT# PABW-088)
Application: ELISA, WB

Figure 4 Human Anti-TSHR Recombinant Antibody (PABL-737) in DB

Human Anti-TSHR Recombinant Antibody (clone K1-70) (CAT# PABL-737)
Application: Block

Figure 2 Anti-Human TEM7R Recombinant Antibody (TAB-577CT) in HPLC

Human Anti-PLXDC2 Recombinant Antibody (TAB-577CT) (CAT# TAB-577CT)
Application: ELISA, FC, IHC, IF

Figure 5 Recombinant Mouse Anti-Integrin alpha 1 Antibody (PABX-121) in ELISA

Recombinant Mouse Anti-Integrin alpha 1 Antibody (AQC2) (CAT# PABX-121)
Application: WB, ELISA, Neut, FuncS

Figure 2 Human CRCP Recombinant Antibody (TAB-426CQ) in HPLC

Human Anti-CRCP Recombinant Antibody (TAB-426CQ) (CAT# TAB-426CQ)
Application: ELISA, IHC, FC, IP, IF, Inhib

Figure 4 Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ, Fc Silenced) in ELISA

Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ) (CAT# TAB-437CQ)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 2 Human Anti-SLC30A8 Recombinant Antibody (clone C22) (HPAB-0040-CN) in SDS-PAGE

Human Anti-SLC30A8 Recombinant Antibody (clone C22) (CAT# HPAB-0040-CN)
Application: ELISA, WB, IHC

Figure 4 Anti-Human CD47 Antibody (HPAB-0008-FY) in HPLC

Human Anti-CD47 Recombinant Antibody (HPAB-0008-FY) (CAT# HPAB-0008-FY)
Application: ELISA, PD, Activ, PK, Stim

Figure 5 Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (HPAB-0330CQ) in DB

Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (CAT# HPAB-0330CQ)
Application: ELISA, IF, Block, FuncS

Figure 3 Anti-C5 Recombinant Antibody (PABC-462) in DB

Human Anti-C5 Recombinant Antibody (PABC-462) (CAT# PABC-462)
Application: WB, HA, ELISA, FC, Neut

Figure 4 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in ELISA

Mouse Anti-MICA/B Recombinant Antibody (PABC-516) (CAT# PABC-516)
Application: ELISA

Figure 5 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in ELISA

Mouse Anti-CRTAM Recombinant Antibody (clone 17B2) (CAT# HPAB-1089-FY)
Application: WB, FC

Figure 3 Mouse Anti-Fibrin β chain Antibody (MOB-195YJL) in DB

Mouse Anti-Fibrin β chain Recombinant Antibody (MOB-195YJL) (CAT# MOB-195YJL)
Application: ELISA

Figure 5 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in Dot Blot

Human Anti-IAV H3N2 HA Recombinant Antibody (clone S5V2-29) (CAT# PABJ-0152)
Application: ELISA, Neut, FuncS

Figure 4 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in DB

Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (CAT# PABJ-0170)
Application: ELISA, Neut

Figure 3 Mouse Anti-SOX9 Antibody (clone ABT-SOX9) in WB

Mouse Anti-SOX9 Recombinant Antibody (clone ABT-SOX9) (CAT# ZG-0104C)
Application: IHC-P, IF

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.