Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv
CAT#: VS-0525-YC202
The Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv was designed as a biparatopic antibody (bpAb) with tandem scFv arms, this construct binds two distinct epitopes on CD274 (PD-L1) with high affinity. One epitope is constituted by the C, F, and G strands, the CC' loop, and the N-terminal region of PD-L1. The other epitope is formed by the CC'FG antiparallel β-sheet and the BC, CC', C''C'', and FG loops.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
Normal Tissue
Figure 5 Placenta
Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274
❮
❯
Specifications
- Host Animal 1
- Human
- Host Animal 2
- Human
- Specificity
- Human CD274
- Species Reactivity
- Human
- Type
- Tandem scFv
- Valency
- 1 + 1
- Epitope 1
- The epitope 1 is constituted by the C strand, F strand, G strand, CC' loop, and N-terminal region of PD-L1.
- Epitope 2
- The epitope 2 is formed by the CC'FG antiparallel β-sheet and the BC, CC', C'C″, and FG loops
- Purity
- >90%
- Purification
- Affinity purified
- Applications
- ELISA, IHC, FC, IP, IF
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid freeze-thaw cycles.
- Long Name
- CD274 Molecule
Target
- Introduction
- This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
- Alternative Names
- Programmed cell death 1 ligand 1; B7H1; B7-H1; PDL1; PD-L1; PDCD1L1; B7 homolog 1; B7 homologue 1
- Gene ID
- 29126
- UniProt ID
- Q9NZQ7
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-305-sdAb | Recombinant Anti-Mouse Cd274 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1814z | Mouse Anti-CD274 Recombinant Antibody (clone 7A5) | FC, IHC, FuncS | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006) | WB, ELISA, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-298 | Human Anti-CD274 Recombinant Antibody (clone CBLC-006); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0190CL | Anti-Human CD274 Recombinant Antibody (PD1-AB1) | FC, ELISA, FuncS |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0262CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0263CL | Human Anti-CD274 Recombinant Antibody (TAB-0263CL) | ELISA, WB, SPR, FuncS | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0264CL | Human Anti-CD274 Recombinant Antibody (TAB-0264CL) | ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0265CL | Human Anti-CD274 Recombinant Antibody (TAB-0265CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0266CL | Human Anti-CD274 Recombinant Antibody (TAB-0266CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0267CL | Human Anti-CD274 Recombinant Antibody (TAB-0267CL) | ELISA, WB, SPR, ADCC, CDC, FuncS | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0992CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL) | Inhib, FC, SPR | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0993CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0993CL) | FC, SPR | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0994CL | Mouse Anti-CD274 Recombinant Antibody (TAB-0994CL) | ELISA, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0995CL | Human Anti-CD274 Recombinant Antibody (TAB-0995CL) | ELISA, FC | Chimeric (Mouse/Human) IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0997CL | Anti-Human CD274 Recombinant Antibody (B60-55) | ELISA, FuncS, FC, PK | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1002CL | Mouse Anti-CD274 Recombinant Antibody (TAB-1002CL) | ELISA, FC | Mouse IgG, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1003CL | Human Anti-CD274 Recombinant Antibody (TAB-1003CL) | FC, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1004CL | Human Anti-CD274 Recombinant Antibody (TAB-1004CL) | FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1009CL | Mouse Anti-CD274 Recombinant Antibody (TAB-1009CL) | WB, IHC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0995CL-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0995CL-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2354MZ | Mouse Anti-CD274 Recombinant Antibody (clone NJI3) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-324CQ | Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6) | IHC-P, FC, Neut, ICC | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-325CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-206) | Neut | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-326CQ | Human Anti-CD274 Recombinant Antibody (clone CBLS-207) | ELISA, Neut | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-327CQ | Mouse Anti-CD274 Recombinant Antibody (clone MIH1) | FC, FuncS, Neut, IHC, IF | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-328CQ | Rat Anti-CD274 Recombinant Antibody (VMC171) | Block, FC | Rat IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-329CQ | Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2) | FC, IF, Block | Rat IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-330CQ | Rat Anti-CD274 Recombinant Antibody (clone MIH5) | FC, FuncS, Neut, IHC-Fr, WB | Rat IgG2a, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-331CQ | Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1) | ELISA, Block | Mouse IgG1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-332CQ | Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009) | Block | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0548 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4049 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4050 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS) | ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4051 | Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0193-YC | Human Anti-CD274 Recombinant Antibody (HPAB-0193-YC) | ELISA | Humanized IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0194-YC | Mouse Anti-CD274 Recombinant Antibody (HPAB-0194-YC) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0193-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0193-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0194-YC-S(P) | Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0194-YC-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0195-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0195-YC-S(P)) | SPR | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0196-YC-S(P) | Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0196-YC-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0193-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0194-YC-F(E) | Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0195-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E)) | SPR | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0196-YC-F(E) | Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0408-YC-VHH | Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0408-YC-VHH) | ELISA, FC, BL | Human VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-417CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-418CQ | Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ) | ELISA, IHC, FC, IP, IF, BL | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0307-YC-VHH | Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH) | ELISA, FC, BL | Humanized VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NBD-0065-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NBD-0066-YJ-VHH | Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH) | ELISA, Inihb | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC110 | Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced | ELISA, WB, SPR, ADCC, CDC | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC111 | Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced | ELISA, WB, SPR, ADCC, CDC, Inhib | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY375 | Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY32 | CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY65 | Human Anti-CD274 (clone MI4736) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC608 | Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608) | Block, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1152 | Anti-CD274 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1153 | Anti-Mouse CD274 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1154 | Anti-Human CD274 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC8 | SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC8) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC47 | SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC47) | ELISA, IHC, FC, IP, IF, Inhib | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC9 | Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC9) | ISZ, Cyt, FuncS |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC39 | Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC39) | ISZ, Cyt, FuncS |
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