Anti-Mouse CD274 Immunohistochemistry Kit

Name: Anti-Mouse CD274 Immunohistochemistry Kit
SKU: VS-0525-XY1153
Rating: 5 (1 reviews)

CAT#: VS-0525-XY1153

The CD274 IHC kit provides a reliable and easy-to-use solution for detecting PD-L1 (CD274) in tissue sections. It is suitable for use with both FFPE and frozen tissues, offering high sensitivity and clear results. The reagents are optimized to ensure reproducible, high-quality staining with minimal background.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Mouse

Target
CD274

Primary Antibody
Rabbit Anti-CD274 Antibody

Secondary Antibody
Goat anti-Rabbit Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Datasheet

Datasheet - VS-0525-XY1153.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0525-XY1153.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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