Anti-Human CD274 Immunohistochemistry Kit

Name: Anti-Human CD274 Immunohistochemistry Kit
SKU: VS-0525-XY1154
Rating: 5 (1 reviews)

CAT#: VS-0525-XY1154

Our CD274 IHC kit offers fully optimized reagents for the accurate detection of PD-L1 expression in various species and tissue types. Suitable for both paraffin and frozen sections, this kit streamlines immunohistochemical workflows, helping researchers achieve reliable results in cancer and immune checkpoint studies.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Human, Monkey

Target
CD274

Primary Antibody
Mouse Anti-CD274 Antibody

Secondary Antibody
Goat anti-Mouse Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Downloadable Resources

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Datasheet

Datasheet - VS-0525-XY1154.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0525-XY1154.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Cd274"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
TAB-0190CL	Anti-Human CD274 Recombinant Antibody (PD1-AB1)	FC, ELISA, FuncS
TAB-0190CL-S(P)	Anti-Human CD274 Recombinant Antibody scFv Fragment (PD1-AB1)	FC, ELISA, FuncS
TAB-1003CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1003CL-F(E))	FC, ELISA	Human Fab
TAB-1004CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1004CL-F(E))	FC	Human Fab
TAB-417CQ	Anti-Human CD274 Recombinant Antibody (TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	IgG1, κ

CAT	Product Name	Application	Type
TAB-0262CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Mouse IgG
TAB-0262CL-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0262CL-S(P))	ELISA, WB, SPR, FuncS	Mouse scFv
TAB-0262CL-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0262CL-F(E))	ELISA, WB, SPR, FuncS	Mouse Fab
TAB-1009CL-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1009CL-F(E))	WB, IHC	Mouse Fab
TAB-1010CL-F(E)	Anti-Human CD274 Recombinant Antibody Fab Fragment	FC

CAT	Product Name	Application	Type
TAB-0263CL	Human Anti-CD274 Recombinant Antibody (TAB-0263CL)	ELISA, WB, SPR, FuncS	Humanized IgG
TAB-0264CL	Human Anti-CD274 Recombinant Antibody (TAB-0264CL)	ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib	Humanized IgG
TAB-0265CL	Human Anti-CD274 Recombinant Antibody (TAB-0265CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG
TAB-0266CL	Human Anti-CD274 Recombinant Antibody (TAB-0266CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG
TAB-0267CL	Human Anti-CD274 Recombinant Antibody (TAB-0267CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

CAT	Product Name	Application	Type
TAB-0995CL	Human Anti-CD274 Recombinant Antibody (TAB-0995CL)	ELISA, FC	Chimeric (Mouse/Human) IgG1, κ
TAB-0995CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P))	ELISA, FC	Human scFv
TAB-0995CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E))	ELISA, FC	Chimeric (Mouse/Human) Fab

CAT	Product Name	Application	Type
NEUT-324CQ	Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6)	IHC-P, FC, Neut, ICC	Mouse IgG2b, κ
NEUT-325CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-206)	Neut	Human IgG1, κ
NEUT-326CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-207)	ELISA, Neut	Human IgG1, κ
NEUT-327CQ	Mouse Anti-CD274 Recombinant Antibody (clone MIH1)	FC, FuncS, Neut, IHC, IF	Mouse IgG1, κ
NEUT-330CQ	Rat Anti-CD274 Recombinant Antibody (clone MIH5)	FC, FuncS, Neut, IHC-Fr, WB	Rat IgG2a, λ

CAT	Product Name	Application	Type
NEUT-328CQ	Rat Anti-CD274 Recombinant Antibody (VMC171)	Block, FC	Rat IgG2
NEUT-329CQ	Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2)	FC, IF, Block	Rat IgG2b, κ
NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ
NEUT-332CQ	Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009)	Block	Rabbit IgG

CAT	Product Name	Application	Type
MOR-0548	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB)	ELISA	Rabbit IgG
MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG
MOR-4050	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS)	ICC, IF, FC	Rabbit IgG
MOR-4051	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS)	FC	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0933LY-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0933LY-S(P))	Inhib, ELISA	Human scfv
HPAB-0934LY-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0934LY-S(P))	Inhib, ELISA	Human scfv
HPAB-0935LY-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0935LY-S(P))	Inhib, ELISA	Human scfv
HPAB-0936LY-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0936LY-S(P))	Inhib, ELISA	Human scfv
HPAB-0937LY-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0937LY-S(P))	Inhib, ELISA	Human scfv

CAT	Product Name	Application	Type
HPAB-0528LY-F(E)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-1); Fab Fragment	ELISA	Human Fab
HPAB-0529LY-F(E)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-2); Fab Fragment	ELISA	Human Fab
HPAB-0530LY-F(E)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-3); Fab Fragment	ELISA	Human Fab
HPAB-0531LY-F(E)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-4); Fab Fragment	ELISA	Human Fab
HPAB-0532LY-F(E)	Human Anti-CD274 Recombinant Antibody (clone 047HAb-5); Fab Fragment	ELISA	Human Fab

CAT	Product Name	Application	Type
HPAB-0114-YJ	Human Anti-CD274 Recombinant Antibody (HPAB-0114-YJ)	Block, ELISA, FC, Neut, FuncS	Human IgG1
HPAB-0115-YJ	Human Anti-CD274 Recombinant Antibody (HPAB-0115-YJ)	Block, ELISA, FC, Neut, FuncS	Human IgG1
HPAB-0116-YJ	Human Anti-CD274 Recombinant Antibody (HPAB-0116-YJ)	ELISA, Block	Human IgG
HPAB-0117-YJ	Human Anti-CD274 Recombinant Antibody (HPAB-0117-YJ)	ELISA, Block	Human IgG
HPAB-M0458-YC	Human Anti-CD274 Recombinant Antibody (HPAB-M0458-YC)	ELISA, FC	Humanized IgG

CAT	Product Name	Application	Type
AFC-TAB-417CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody
AFC-TAB-418CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody

CAT	Product Name	Application	Type
NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH
NBD-0066-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH)	ELISA, Inihb	Camelid VHH
NBD-0067-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0067-YJ-VHH)	ELISA, FC	Camelid VHH
NBD-0068-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0068-YJ-VHH)	ELISA, FC	Camelid VHH
NBD-0069-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0069-YJ-VHH)	ELISA, FC	Camelid VHH

CAT	Product Name	Application	Type
VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer
VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

CAT	Product Name	Application	Type
VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0325-FY65	Human Anti-CD274 (clone MI4736) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC
VS-0525-XY1153	Anti-Mouse CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

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