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    Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced (CAT#: VS-0924-YC110)

    The product is a hexameric mouse anti-CD274 antibody with enhanced complement-dependent cytotoxicity (CDC) activity. This hexameric IgG1 targets human CD274 and includes hexamerization-enhancing mutations that promote hexamer formation, thereby facilitating optimal C1q binding and boosting CDC.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Fc Engineering:

    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg
    Figure 2 IF staining of human cell line BJ
    Figure 2 IF staining of human cell line BJ
    (Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg
    Normal Tissue
    Figure 4 Colon
    Figure 4 Colon
    (Peripheral nerve/ganglion
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg
    Figure 5 Placenta
    Figure 5 Placenta
    (Cytotrophoblasts
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Syncytiotrophoblasts - cell body
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Syncytiotrophoblasts - microvilli
    Staining:High
    Intensity: Strong)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg
    RNA Expression
    Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
    Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

    Specifications

    • Host Species
    • Mouse
    • Type
    • Antibody hexamer
    • Specificity
    • Human CD274
    • Species Reactivity
    • Human
    • Molecule Class
    • ~ 900 kDa
    • Applications
    • ELISA, WB, SPR, ADCC, CDC
    • Conjugate
    • None
    • Related Disease
    • Cancer, infectious diseases, pathological disorders

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein G purified
    • Purity
    • >95% determined as SDS-PAGE and SEC-HPLC
    • Aggregation
    • <5%
    • Buffer
    • PBS, pH 7.4
    • Preservative
    • No preservatives
    • Storage
    • Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • CD274; CD274 molecule; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
    • Long Name
    • CD274 Molecule

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    CAT Product Name Application Type
    NAB-305-sdAb Recombinant Anti-Mouse Cd274 VHH Single Domain Antibody IHC, IP, FC, Neut, FUNC Llama VHH
    HPAB-N0307-YC-VHH Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH) ELISA, FC, BL Humanized VHH
    NBD-0065-YJ-VHH Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH) ELISA Camelid VHH
    NBD-0066-YJ-VHH Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH) ELISA, Inihb Camelid VHH
    NBD-0067-YJ-VHH Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0067-YJ-VHH) ELISA, FC Camelid VHH

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