Anti-ERBB3 Immunohistochemistry Kit

Name: Anti-ERBB3 Immunohistochemistry Kit
SKU: VS-0525-XY2337
Rating: 5 (1 reviews)

CAT#: VS-0525-XY2337

The ERBB3 IHC kit provides complete reagents for accurate detection of ERBB3 in tissue sections. It is optimized for both paraffin and frozen tissue sections, ensuring high reproducibility. The optimized protocol ensures high sensitivity with minimal background interference.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg

Normal Tissue Figure 8 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg

Normal Tissue Figure 11 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg

Normal Tissue Figure 12 Skin

Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg

Normal Tissue Figure 13 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg

RNA Expression Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Human

Target
ERBB3

Primary Antibody
Mouse Anti-ERBB3 Antibody

Secondary Antibody
Goat anti-Mouse Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - VS-0525-XY2337.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0525-XY2337.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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