SmartAb™ Recombinant Anti-ERBB3 pH-dependent Antibody (Clone Seribantumab)

Name: SmartAb™ Recombinant Anti-ERBB3 pH-dependent Antibody (Clone Seribantumab)
SKU: VS-0825-YC118
Rating: 5 (1 reviews)

CAT#: VS-0825-YC118

The anti-ERBB3 pH-dependent antibody (clone Seribantumab) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human ERBB3. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for ERBB3 under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from ERBB3 at pH 6.0. This pH-sensitive antibody retains its binding affinity for ERBB3 on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human ERBB3, also known as HER3, is a member of the EGFR family of receptor tyrosine kinases. Unlike its counterparts, it has very low intrinsic kinase activity and primarily acts as a co-receptor, signaling through heterodimerization with other family members.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg

Normal Tissue Figure 8 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg

Normal Tissue Figure 11 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg

Normal Tissue Figure 12 Skin

Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg

Normal Tissue Figure 13 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg

RNA Expression Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

❮

❯

Specifications

Host Species
Human

Derivation
Humanized

Type
Human IgG2 lambda

Specificity
Human ERBB3

Species Reactivity
Human

Clone
Seribantumab

Applications
ELISA, FC, ICC, IF, IP, Neut

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE and SEC-HPLC

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.

Target

Alternative Names
ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); LCCS2, lethal congenital contracture syndrome 2; receptor tyrosine-protein kinase erbB-3; HER3; proto-oncogene-like protein c-ErbB-3; tyrosine k

Gene ID
2065

UniProt ID
P21860

Long Name
Erb-B2 Receptor Tyrosine Kinase 3

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for VS-0825-YC118. Click the button above to contact us or submit your feedback about this product.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0825-YC118.pdf

MSDS

MSDS - VS-0825-YC118.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "ERBB3"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
NAB-1729-sdAb	Recombinant Anti-human ERBB3 VHH Single Domain Antibody	WB, ICC, ChiP, FA, ELISA	Llama VHH
TAB-070CT	Llama Anti-ERBB3 Recombinant Single Domain Antibody (TAB-070CT)	FC, Block	Llama VHH
PABC-556	Recombinant Llama Anti-ERBB3 Single Domain Antibody (PABC-556)	ELISA, SPR	Llama VHH

CAT	Product Name	Application	Type
TAB-189	Human Anti-ERBB3 Recombinant Antibody (TAB-189)	IP, IF, FuncS, FC, Neut, ELISA, ICC	Human IgG1, κ
TAB-0033CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0033CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0034CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0034CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0035CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0035CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0036CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0036CL)	ELISA, FuncS, Inhib, FC	Human IgG

CAT	Product Name	Application	Type
TAB-892	Anti-Human ERBB3/ErbB 3 Recombinant Antibody (Seribantumab)	IF, IP, Neut, FuncS, ELISA, FC, ICC	IgG2 - lambda
TAB-H22	Anti-Human ERBB3 Recombinant Antibody (Duligotuzumab) (TAB-H22)	ELISA, IP, FC, FuncS, Neut, IF, ICC	IgG1 - kappa
TAB-057CT-F(E)	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-057CT-F(E))	Inhibion, ELISA	Humanized Fab
TAB-058CT-F(E)	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-058CT-F(E))	WB, ELISA, Inhib	Humanized Fab
TAB-063CT-F(E)	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-063CT-F(E))	ELISA, Inhib, FC	Humanized Fab

CAT	Product Name	Application	Type
AGTO-L074E	HRGβ2-PE immunotoxin	Cytotoxicity assay, Functional assay

CAT	Product Name	Application	Type
PFBL-127	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (PFBL-127)	ELISA, WB, FuncS	Human Fab
PFBL-128	Mouse Anti-ERBB3 Recombinant Antibody; Fab Fragment (PFBL-128)	WB, Block, FuncS	Mouse Fab
HPAB-0724-FY-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone AB062); Fab Fragment	ELISA	Mouse Fab
HPAB-0783-FY-S(P)	Human Anti-ERBB3 Recombinant Antibody (clone Hu-Lc9); Fab Fragment	ELISA	Human Fab
HPAB-0784-FY-S(P)	Human Anti-ERBB3 Recombinant Antibody (clone Hu-2Hl); Fab Fragment	ELISA	Human Fab

CAT	Product Name	Application	Type
TAB-0552CL	Mouse Anti-ERBB3 Recombinant Antibody (TAB-0552CL)	ELISA, Inhib	Mouse IgG
TAB-0553CL	Mouse Anti-ERBB3 Recombinant Antibody (TAB-0553CL)	ELISA, Inhib	Mouse IgG
TAB-0508CL-S(P)	Anti-Human ERBB3 Recombinant Antibody scFv Fragment (1A5)	ELISA, Inhib, FuncS
TAB-0509CL-S(P)	Anti-Human ERBB3 Recombinant Antibody scFv Fragment (3D4)	ELISA, Inhib, FuncS
TAB-0552CL-S(P)	Mouse Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-0552CL-S(P))	ELISA, Inhib	Mouse scFv

CAT	Product Name	Application	Type
TAB-061CT	Human Anti-ERBB3 Recombinant Antibody (TAB-061CT)	Inhib, ELISA	Chimeric (Rabbit/Human) antibody
TAB-061CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-061CT-S(P))	Inhib, ELISA	Human scFv
TAB-061CT-F(E)	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-061CT-F(E))	Inhib, ELISA	Chimeric (Rabbit/Human) Fab

CAT	Product Name	Application	Type
NEUT-738CQ	Mouse Anti-ERBB3 Recombinant Antibody (clone CBL932)	Neut, IP	Mouse IgG1
NEUT-739CQ	Mouse Anti-ERBB3 Recombinant Antibody (clone CBL483)	FC, CyTOF®, ELISA, Neut	Mouse IgG1
NEUT-740CQ	Human Anti-ERBB3 Recombinant Antibody (clone CBL1019)	Neut	Human IgG1, κ
NEUT-741CQ	Human Anti-ERBB3 Recombinant Antibody (clone CBL1020)	Neut	Human IgG1, κ
NEUT-742CQ	Mouse Anti-ERBB3 Recombinant Antibody (NEUT-742CQ)	Neut	Mouse IgG1

CAT	Product Name	Application	Type
MOR-1179	Hi-Affi™ Rabbit Anti-ERBB3 Recombinant Antibody (clone DS1179AB)	ICC, IF, WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0286-YC-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone 1153); scFv Fragment	ELISA, FuncS, FC	Mouse scFv
HPAB-0287-YC-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone 920104); scFv Fragment	ELISA, FuncS, FC	Mouse scFv
HPAB-0288-YC-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone 1126); scFv Fragment	ELISA, FuncS, FC	Mouse scFv
HPAB-0289-YC-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone 12511); scFv Fragment	ELISA, FuncS, FC	Mouse scFv
HPAB-0290-YC-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (HPAB-0290-YC-S(P))	ELISA, FuncS, FC	Human scFv

CAT	Product Name	Application	Type
HPAB-0081-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0081-FY)	Inhib, FuncS	Humanized IgG1, κ
HPAB-0120-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0120-FY)	ELISA, WB	Humanized IgG1, κ
HPAB-0128-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0128-FY)	ELISA	Humanized IgG1, κ
HPAB-0181-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0181-FY)	ELISA, FC	Human IgG1
HPAB-0182-FY	Human Anti-ERBB3 Recombinant Antibody (HPAB-0182-FY)	ELISA, FC	Human IgG

CAT	Product Name	Application	Type
AFC-TAB-H21	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H21)	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody
AFC-TAB-422CQ	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-422CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody
AFC-TAB-189	Afuco™ Anti-ERBB3 Recombinant Antibody (AFC-TAB-189), ADCC Enhanced	IP, IF, FuncS, FC, Neut, ELISA	Human IgG1, κ
AFC-TAB-H22	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H22)	ELISA, IP, FC, FuncS, Neut, IF	ADCC enhanced antibody
AFC-TAB-892	Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-892)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0424-XY92	AbPlus™ Anti-ERBB3 Magnetic Beads (Duligotuzumab)	IP, Protein Purification
VS-0724-YC1400	AbPlus™ Anti-ERBB3 Magnetic Beads (VS-0724-YC1400)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0125-FY22	Human Anti-ERBB3 (clone H3) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc
VS-0125-FY23	Human Anti-ERBB3 (clone PM6) scFv-Fc Chimera	WB, ELISA, IHC, FuncS, BI	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC478	Recombinant Anti-ERBB3 Vesicular Antibody, EV Displayed (VS-0425-YC478)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY2337	Anti-ERBB3 Immunohistochemistry Kit	IHC
VS-0525-XY2338	Anti-Mouse ERBB3 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC72	Recombinant Anti-ERBB3 (Domain 1 x Domain 3) Biparatopic Antibody, Tandem scFv (Clone 1153 x Clone 920104)	ELISA, FC	Tandem scFv
VS-0525-YC74	Recombinant Anti-ERBB3 (Domain 3 x Domain 4) Biparatopic Antibody, Tandem scFv (Clone 920104 x Clone 1126)	ELISA, FC	Tandem scFv

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 1 Anti-P. aeruginosa psl Antibody in SDS-PAGE

Recombinant Anti-P. aeruginosa psl Antibody (CAT# MOB-736)
Application: WB, IP, FuncS

Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-097) in DB

Anti-Human IL6 Recombinant Antibody (Sirukumab) (CAT# TAB-097)
Application: FC, IP, ELISA, Neut, FuncS, IF, WB

Figure 5 GPNMB Recombinant Antibody (TAB-751) in ELISA

Human Anti-GPNMB Recombinant Antibody (clone Glembatumumab) (CAT# TAB-751)
Application: WB, IF, IP, Neut, FuncS, ELISA, FC

Figure 2 Anti-Human CD25 Antibody (TAB-023) in HPLC

Anti-Human CD25 Recombinant Antibody (Daclizumab) (CAT# TAB-023)
Application: ELISA, Neut, IF, IP, FC, FuncS

Figure 1 Anti-Human EGFR Antibody in ELISA

Anti-Human EGFR Recombinant Antibody (TAB-119) (CAT# TAB-119)
Application: FC, IP, ELISA, Neut, FuncS, IF, WB

Figure 1 Anti-Human IL17RA Recombinant Antibody (TAB-217) in SDS-PAGE

Anti-Human IL17RA Recombinant Antibody (TAB-217) (CAT# TAB-217)
Application: WB, ELISA, FC, IP, FuncS, IF, Neut

Figure 2 Anti-Human CD24 Antibody (TAB-113CL) in WB

Anti-Human CD24 Recombinant Antibody (CAT# TAB-113CL)
Application:

Figure 4 Afuco™ Anti-Human LRRC15 ADCC Recombinant Antibody, ADCC Enhanced (AFC-480CL) in Lectin ELISA

Afuco™ Anti-Human LRRC15 ADCC Recombinant Antibody, ADCC Enhanced (CAT# AFC-480CL)
Application:

Figure 3 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in DB

Mouse Anti-Il2 Recombinant Antibody (PABL-609) (CAT# PABL-609)
Application: FC, FuncS, IHC, ELISA

Figure 2 Anti-LPA Recombinant Antibody (TAB-0340CL) in HPLC

Anti-LPA Recombinant Antibody (LT3015 [B7(LT3000, Lpathomab)]) (CAT# TAB-0340CL)
Application: ELISA, SPR, Inhib, FuncS

Figure 3 Anti-FOLR1 Antibody (TAB-020CQ) in Dot Blot

Mouse Anti-FOLR1 Recombinant Antibody (TAB-020CQ) (CAT# TAB-020CQ)
Application: ELISA, FC, WB, IHC

Figure 3 Anti-Human IgE Recombinant Antibody Fab Fragment (CMAB007) in ELISA.

Anti-Human IgE Recombinant Antibody Fab Fragment (CMAB007) (CAT# TAB-034ZJ-F(E))
Application: ELISA

Figure 3 Anti-Human IgE Antibody (TAB-095ZJ) in ELISA

Anti-Human IgE Single Domain Antibody (TAB-095ZJ), Research Grade Biosimilar (CAT# TAB-095ZJ)
Application: ELISA, Inhib, FC

Figure 5 Anti-Human Tau Recombinant Antibody (TAB-528CT) in ELISA

Anti-Human Tau Recombinant Antibody (ADx202) (CAT# TAB-528CT)
Application: WB, ELISA, IHC

Figure 2 Anti-Human PLAUR Recombinant Antibody (TAB-238CQ) in HPLC

Anti-Human PLAUR Recombinant Antibody (2.19.2) (CAT# TAB-238CQ)
Application: Inhib, WB

Figure 2 Anti-Human SLC34A2 Recombinant Antibody (TAB-467MZ) in HPLC

Mouse Anti-SLC34A2 Recombinant Antibody (TAB-467MZ) (CAT# TAB-467MZ)
Application: FC, Inhib

Figure 8 Anti-IAV HA Recombinant Antibody (MRO-276CT) in DB

Anti-IAV HA Recombinant Antibody (12D1) (CAT# MRO-276CT)
Application: ELISA, WB, Neut, Funcs, IHC, Inhib

Figure 4 Anti-Human SNCA Antibody (TAB-0750CLV) in HPLC

Mouse Anti-SNCA Recombinant Antibody (TAB-0750CLV) (CAT# TAB-0750CLV)
Application: ELISA

Figure 4 Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ, Fc Silenced) in ELISA

Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ) (CAT# TAB-437CQ)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 4 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in ELISA

Mouse Anti-MICA/B Recombinant Antibody (PABC-516) (CAT# PABC-516)
Application: ELISA

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.