Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H22)

Name: Afuco™ Anti-ERBB3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H22)
SKU: AFC-TAB-H22
Rating: 5 (1 reviews)

CAT#: AFC-TAB-H22

This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant Humanized antibody to Human ERBB3

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg

Normal Tissue Figure 8 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg

Normal Tissue Figure 11 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg

Normal Tissue Figure 12 Skin

Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg

Normal Tissue Figure 13 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg

RNA Expression Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

❮

❯

Specifications

Host Species
Human

Derivation
Humanized

Type
ADCC enhanced antibody

Specificity
ERBB3 (receptor tyrosine-protein kinase erbB-3, HER3) [Homo sapiens]

Species Reactivity
Human

Applications
ELISA, IP, FC, FuncS, Neut, IF

Conjugate
Unconjugated

Generic Name
Duligotuzumab

Related Disease
Cancers

Product Property

Purity
>95% by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); LCCS2, lethal congenital contracture syndrome 2; receptor tyrosine-protein kinase erbB-3; HER3; proto-oncogene-like protein c-ErbB-3;

Gene ID
2065

UniProt ID
P21860

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Biosimilar Overview

Please refer to Duligotuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Duligotuzumab.

Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AFC-TAB-H22.pdf

MSDS

MSDS - AFC-TAB-H22.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "duligotuzumab"

Anti-Human ERBB3 Recombinant Antibody (Duligotuzumab) (TAB-H22)
Recombinant Humanized antibody to Human ERBB3

DrugMonitor™ Anti-Duligotuzumab Antibody (VS-1224-YC379)
Duligotuzumab has been studied for treating neoplasms, colorectal cancer, head and neck cancer, and malignant epithelial tumors. The DrugMonitor™ Anti-Duligotuzumab Antibody (VS-1224-YC379) is an anti-drug antibody (ADA) against Duligotuzumab. This drug-based antibody is raised in mice immunized with the Duligotuzumab. The anti-Duligotuzumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Duligotuzumab in samples.

See other products for "ERBB3"

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CAT	Product Name	Application	Type
MOB-1319z	Mouse Anti-ERBB3 Recombinant Antibody (clone 22C5)	ELISA, ICC, IF, WB	Mouse IgG1
MOB-0309MZ	Recombinant Mouse Anti-Human ERBB3 Antibody (clone EAK-H4-IC)	IHC	Mouse antibody
HPAB-AP084-YC	Human Anti-ERBB3 Recombinant Antibody (HPAB-AP084-YC)	ELISA, FuncS	Human IgG
HPAB-AP085-YC	Human Anti-ERBB3 Recombinant Antibody (HPAB-AP085-YC)	ELISA, FuncS	Human IgG
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CAT	Product Name	Application	Type
TAB-189	Human Anti-ERBB3 Recombinant Antibody (TAB-189)	IP, IF, FuncS, FC, Neut, ELISA, ICC	Human IgG1, κ
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TAB-0034CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0034CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0035CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0035CL)	ELISA, FuncS, Inhib, FC	Human IgG
TAB-0036CL	Human Anti-ERBB3 Recombinant Antibody (TAB-0036CL)	ELISA, FuncS, Inhib, FC	Human IgG

CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
PSBL-086	Human Anti-ERBB3 Recombinant Antibody (clone KTN3379); scFv Fragment	ELISA, WB, IF, FuncS	Human scFv
PSBL-127	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (PSBL-127)	ELISA, WB, FuncS	Human scFv
PSBL-128	Mouse Anti-ERBB3 Recombinant Antibody; scFv Fragment (PSBL-128)	WB, Block, FuncS	Mouse scFv
HPAB-0171-WJ-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (HPAB-0171-WJ-S(P))	ELISA	Human scFv
HPAB-0200-WJ-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone 205.10.1); scFv Fragment	ELISA, WB	Mouse scFv

CAT	Product Name	Application	Type
PFBL-127	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (PFBL-127)	ELISA, WB, FuncS	Human Fab
PFBL-128	Mouse Anti-ERBB3 Recombinant Antibody; Fab Fragment (PFBL-128)	WB, Block, FuncS	Mouse Fab
HPAB-0724-FY-S(P)	Mouse Anti-ERBB3 Recombinant Antibody (clone AB062); Fab Fragment	ELISA	Mouse Fab
HPAB-0783-FY-S(P)	Human Anti-ERBB3 Recombinant Antibody (clone Hu-Lc9); Fab Fragment	ELISA	Human Fab
HPAB-0784-FY-S(P)	Human Anti-ERBB3 Recombinant Antibody (clone Hu-2Hl); Fab Fragment	ELISA	Human Fab

CAT	Product Name	Application	Type
TAB-0552CL-S(P)	Mouse Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-0552CL-S(P))	ELISA, Inhib	Mouse scFv
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TAB-0509CL-F(E)	Anti-Human ERBB3 Recombinant Antibody Fab Fragment (3D4)	ELISA, Inhib, FuncS
TAB-060CT-S(P)	Rabbit Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-060CT-S(P))	Inhib, ELISA	Rabbit scFv
TAB-064CT-S(P)	Mouse Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-064CT-S(P))	ELISA, FC	Mouse scFv

CAT	Product Name	Application	Type
TAB-061CT	Human Anti-ERBB3 Recombinant Antibody (TAB-061CT)	Inhib, ELISA	Chimeric (Rabbit/Human) antibody
TAB-061CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-061CT-S(P))	Inhib, ELISA	Human scFv
TAB-061CT-F(E)	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-061CT-F(E))	Inhib, ELISA	Chimeric (Rabbit/Human) Fab

CAT	Product Name	Application	Type
TAB-057CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-057CT-S(P))	Inhibion, ELISA	Humanized scFv
TAB-058CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-058CT-S(P))	WB, ELISA, Inhib	Human scFv
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TAB-063CT-S(P)	Human Anti-ERBB3 Recombinant Antibody; scFv Fragment (TAB-063CT-S(P))	ELISA, Inhib, FC	Human scFv
TAB-063CT-F(E)	Human Anti-ERBB3 Recombinant Antibody; Fab Fragment (TAB-063CT-F(E))	ELISA, Inhib, FC	Humanized Fab

CAT	Product Name	Application	Type
NEUT-738CQ	Mouse Anti-ERBB3 Recombinant Antibody (clone CBL932)	Neut, IP	Mouse IgG1
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NEUT-740CQ	Human Anti-ERBB3 Recombinant Antibody (clone CBL1019)	Neut	Human IgG1, κ
NEUT-741CQ	Human Anti-ERBB3 Recombinant Antibody (clone CBL1020)	Neut	Human IgG1, κ
NEUT-742CQ	Mouse Anti-ERBB3 Recombinant Antibody (NEUT-742CQ)	Neut	Mouse IgG1

CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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AFC-TAB-189	Afuco™ Anti-ERBB3 Recombinant Antibody (AFC-TAB-189), ADCC Enhanced	IP, IF, FuncS, FC, Neut, ELISA	Human IgG1, κ
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VS-0125-FY22	Human Anti-ERBB3 (clone H3) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc
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CAT	Product Name	Application	Type
VS-0425-YC478	Recombinant Anti-ERBB3 Vesicular Antibody, EV Displayed (VS-0425-YC478)	ELISA, FC, Neut, Cell-uptake

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