What applications is this antibody recommended for?

We have validated this antibody for use in Enzyme-linked Immunosorbent Assay (ELISA) and Functional Studies (FuncS). It is a reliable tool for investigating ERBB3-mediated signaling.

Human Anti-ERBB3 Recombinant Antibody (HPAB-AP084-YC)

CAT#: HPAB-AP084-YC

This product is a recombinant human antibody that recognizes ERBB3. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70524/162441_A_4_2_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in renal tubular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_8_5_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/659_B11_4_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/658_B11_1_blue_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_7_5.jpg

Normal Tissue Figure 8 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25331/67596_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_5.jpg

Normal Tissue Figure 11 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_4_6.jpg

Normal Tissue Figure 12 Skin

Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Langerhans
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/115200_B_8_1.jpg

Normal Tissue Figure 13 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45396/114856_A_9_8.jpg

RNA Expression Figure 14 RNA cell line category: Cell line enhanced (Hep G2, OE19, RT4, SK-BR-3, SK-MEL-30, T-47d, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000065361-ERBB3

❮

❯

Specifications

Host Species
Human

Derivation
Humanized

Type
Human IgG

Specificity
Human ERBB3

Species Reactivity
Human

Applications
ELISA, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Erb-B2 Receptor Tyrosine Kinase 2; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2; Tyrosine Kinase-Type Cell Surface Receptor HER2; Neuro/Glioblastoma Derived Oncogene Homolog; Human Epidermal Growth Factor Receptor 2; Metastatic Lymph Node Gene 19 Protein; Proto-Oncogene C-ErbB-2; Proto-Oncogene Neu; EC 2.7.10.1; P185erbB2; MLN 19; HER2; NGL; NEU; V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog)

Gene ID
2065

UniProt ID
P21860

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Breast cancer biomarkers at key points during disease progression

Specific for Human ERBB3

This recombinant human antibody against ERBB3 performs very well in ELISA. It specifically recognizes the target antigen expressed in mammalian cells. The affinity purification ensures high quality, and we have seen consistent results in our functional studies.

Great for Functional Assays

We incorporated this antibody into our functional assays to study ERBB3 signaling. It binds tightly and works as expected. The product was delivered with high purity (>95%), and the lack of preservatives allows us to use it in cell-based assays.

Q&As

How is this antibody purified and what is the host?

A: This is a recombinant human antibody (IgG) that recognizes ERBB3. It is expressed in mammalian cells with chemically defined culture media and purified by affinity chromatography to>95% purity.
What applications is this antibody recommended for?

A: We have validated this antibody for use in Enzyme-linked Immunosorbent Assay (ELISA) and Functional Studies (FuncS). It is a reliable tool for investigating ERBB3-mediated signaling.

View the frequently asked questions answered by Creative Biolabs Support.

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Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Related Signaling Pathways

ErbB Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-AP084-YC.pdf

MSDS

MSDS - HPAB-AP084-YC.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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